Characterization of the honeybee AmNa<inf>V</inf>1 channel and tools to assess the toxicity of insecticides
Genre
Journal ArticleDate
2015-07-23Author
Gosselin-Badaroudine, PMoreau, A
Delemotte, L
Cens, T
Collet, C
Rousset, M
Charnet, P
Klein, ML
Chahine, M
Subject
Amino Acid SequenceAnimals
Bees
Binding Sites
Insecticides
Ion Channel Gating
Molecular Docking Simulation
Molecular Sequence Data
Protein Binding
Protein Conformation
Sodium Channel Blockers
Toxicity Tests
Voltage-Gated Sodium Channels
Permanent link to this record
http://hdl.handle.net/20.500.12613/5215
Metadata
Show full item recordDOI
10.1038/srep12475Abstract
Pollination is important for both agriculture and biodiversity. For a significant number of plants, this process is highly, and sometimes exclusively, dependent on the pollination activity of honeybees. The large numbers of honeybee colony losses reported in recent years have been attributed to colony collapse disorder. Various hypotheses, including pesticide overuse, have been suggested to explain the disorder. Using the Xenopus oocytes expression system and two microelectrode voltage-clamp, we report the functional expression and the molecular, biophysical, and pharmacological characterization of the western honeybee's sodium channel (Apis Mellifera NaV1). The NaV1 channel is the primary target for pyrethroid insecticides in insect pests. We further report that the honeybee's channel is also sensitive to permethrin and fenvalerate, respectively type I and type II pyrethroid insecticides. Molecular docking of these insecticides revealed a binding site that is similar to sites previously identified in other insects. We describe in vitro and in silico tools that can be used to test chemical compounds. Our findings could be used to assess the risks that current and next generation pesticides pose to honeybee populations.Citation to related work
Springer Science and Business Media LLCHas part
Scientific ReportsADA compliance
For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.eduae974a485f413a2113503eed53cd6c53
http://dx.doi.org/10.34944/dspace/5197