Characterizing selective pressures on the pathway for de novo biosynthesis of pyrimidines in yeast
Genre
Journal ArticleDate
2015-10-28Author
Hermansen, RAMannakee, BK
Knecht, W
Liberles, DA
Gutenkunst, RN
Subject
Evolutionary systems biologyMetabolic pathway evolution
Phylogenetics
Kinetic model
Enzyme evolution
Substitution rate
Permanent link to this record
http://hdl.handle.net/20.500.12613/5185
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Show full item recordDOI
10.1186/s12862-015-0515-xAbstract
© 2015 Hermansen et al. Background: Selection on proteins is typically measured with the assumption that each protein acts independently. However, selection more likely acts at higher levels of biological organization, requiring an integrative view of protein function. Here, we built a kinetic model for de novo pyrimidine biosynthesis in the yeast Saccharomyces cerevisiae to relate pathway function to selective pressures on individual protein-encoding genes. Results: Gene families across yeast were constructed for each member of the pathway and the ratio of nonsynonymous to synonymous nucleotide substitution rates (dN/dS) was estimated for each enzyme from S. cerevisiae and closely related species. We found a positive relationship between the influence that each enzyme has on pathway function and its selective constraint. Conclusions: We expect this trend to be locally present for enzymes that have pathway control, but over longer evolutionary timescales we expect that mutation-selection balance may change the enzymes that have pathway control.Citation to related work
Springer Science and Business Media LLCHas part
BMC Evolutionary BiologyADA compliance
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http://dx.doi.org/10.34944/dspace/5167