Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
Genre
Journal ArticleDate
2016-08-25Author
Mudumbi, KCSchirmer, EC
Yang, W
Subject
Biological TransportEndoplasmic Reticulum
Feasibility Studies
Fluorescence Recovery After Photobleaching
HeLa Cells
Humans
Membrane Proteins
Microscopy, Confocal
Microscopy, Fluorescence
Nuclear Envelope
Single Molecule Imaging
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http://hdl.handle.net/20.500.12613/5031
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10.1038/ncomms12562Abstract
© The Author(s) 2016. The normal distribution of nuclear envelope transmembrane proteins (NETs) is disrupted in several human diseases. NETs are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM). Quantitative determination of the distribution of NETs on the ONM and INM is limited in available approaches, which moreover provide no information about translocation rates in the two membranes. Here we demonstrate a single-point single-molecule FRAP microscopy technique that enables determination of distribution and translocation rates for NETs in vivo.Citation to related work
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http://dx.doi.org/10.34944/dspace/5013