Internal epitope tagging informed by relative lack of sequence conservation
dc.creator | Burg, L | |
dc.creator | Zhang, K | |
dc.creator | Bonawitz, T | |
dc.creator | Grajevskaja, V | |
dc.creator | Bellipanni, G | |
dc.creator | Waring, R | |
dc.creator | Balciunas, D | |
dc.date.accessioned | 2021-01-25T21:44:26Z | |
dc.date.available | 2021-01-25T21:44:26Z | |
dc.date.issued | 2016-11-28 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.doi | http://dx.doi.org/10.34944/dspace/4975 | |
dc.identifier.other | 27892520 (pubmed) | |
dc.identifier.uri | http://hdl.handle.net/20.500.12613/4993 | |
dc.description.abstract | Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function. | |
dc.format.extent | 36986- | |
dc.language.iso | en | |
dc.relation.haspart | Scientific Reports | |
dc.relation.isreferencedby | Springer Science and Business Media LLC | |
dc.rights | CC BY | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Amino Acid Sequence | |
dc.subject | Animals | |
dc.subject | Antibodies | |
dc.subject | Conserved Sequence | |
dc.subject | Epitopes | |
dc.subject | Proteins | |
dc.subject | Sequence Alignment | |
dc.subject | Zebrafish | |
dc.title | Internal epitope tagging informed by relative lack of sequence conservation | |
dc.type | Article | |
dc.type.genre | Journal Article | |
dc.relation.doi | 10.1038/srep36986 | |
dc.ada.note | For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu | |
dc.creator.orcid | Balciunas, Darius|0000-0003-1938-3243 | |
dc.date.updated | 2021-01-25T21:44:21Z | |
refterms.dateFOA | 2021-01-25T21:44:26Z |