Internal epitope tagging informed by relative lack of sequence conservation
Genre
Journal ArticleDate
2016-11-28Author
Burg, LZhang, K
Bonawitz, T
Grajevskaja, V
Bellipanni, G
Waring, R
Balciunas, D
Subject
Amino Acid SequenceAnimals
Antibodies
Conserved Sequence
Epitopes
Proteins
Sequence Alignment
Zebrafish
Permanent link to this record
http://hdl.handle.net/20.500.12613/4993
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Show full item recordDOI
10.1038/srep36986Abstract
Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function.Citation to related work
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http://dx.doi.org/10.34944/dspace/4975