Internal epitope tagging informed by relative lack of sequence conservation
SubjectAmino Acid Sequence
Permanent link to this recordhttp://hdl.handle.net/20.500.12613/4993
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AbstractMany experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function.
Citation to related workSpringer Science and Business Media LLC
Has partScientific Reports
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