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dc.creatorPalumbo, P
dc.creatorMiconi, G
dc.creatorCinque, B
dc.creatorLombardi, F
dc.creatorTorre, CL
dc.creatorDehcordi, SR
dc.creatorGalzio, R
dc.creatorCimini, A
dc.creatorGiordano, A
dc.creatorCifone, MG
dc.date.accessioned2021-01-25T20:56:23Z
dc.date.available2021-01-25T20:56:23Z
dc.date.issued2017-01-01
dc.identifier.issn1949-2553
dc.identifier.issn1949-2553
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/4956
dc.identifier.otherES2EU (isidoc)
dc.identifier.other28424427 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/4974
dc.description.abstractNitric oxide has been implicated in biology and progression of glioblastoma (GBM) being able to influence the cellular signal depending on the concentration and duration of cell exposure. NOS2 (inducible nitric oxide synthase) have been proposed as a component of molecular profile of several tumors, including glioma, one of the most aggressive primary brain tumor featuring local cancer stem cells responsible for enhanced resistance to therapies and for tumor recurrence. Here, we investigated the NOS2 mRNA expression by reverse transcription-PCR in human glioma primary cultures at several grade of malignancy and glioma stem cell (GSC) derived neurospheres. Glioma cell lines were used as positive controls both in terms of stemness marker expression that of capacity of generating neurospheres. NOS2 expression was detected at basal levels in cell lines and primary cultures and appeared significantly up-regulated in cultures kept in the specific medium for neurospheres. The immunofluorescence analysis of all cell cultures to evaluate the levels of SOX-2, a stemness marker aberrantly up-regulated in GBM, was also performed. The potential correlation between NOS2 expression and ability to generate neurospheres and between NOS2 and SOX-2 levels was also verified. The results show that the higher NOS2 expression is detected in all primary cultures able to arise neurosphere. A high and significant correlation between NOS2 expression and SOX-2 positive cells (%) in all cell cultures maintained in standard conditions has been observed. The results shed light on the potential relevance of NOS2 as a prognostic factor for glioma malignancy and recurrence.
dc.format.extent25582-25598
dc.language.isoen
dc.relation.haspartOncotarget
dc.relation.isreferencedbyImpact Journals, LLC
dc.rightsCC BY
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subjectglioma
dc.subjectprimary cultures
dc.subjectcancer stem cell
dc.subjectNOS2
dc.subjectSOX-2
dc.titleNOS2 expression in glioma cell lines and glioma primary cell cultures: Correlation with neurosphere generation and SOX-2 expression
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.18632/oncotarget.16106
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.creator.orcidGiordano, Antonio|0000-0002-5959-016X
dc.date.updated2021-01-25T20:56:18Z
refterms.dateFOA2021-01-25T20:56:24Z


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