Show simple item record

dc.creatorLuo, W
dc.creatorRuba, A
dc.creatorTakao, D
dc.creatorZweifel, LP
dc.creatorLim, RYH
dc.creatorVerhey, KJ
dc.creatorYang, W
dc.date.accessioned2021-01-22T14:45:00Z
dc.date.available2021-01-22T14:45:00Z
dc.date.issued2017-12-01
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/4833
dc.identifier.other29150645 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/4851
dc.description.abstract© 2017 The Author(s). Transport of membrane and cytosolic proteins in primary cilia is thought to depend on intraflagellar transport (IFT) and diffusion. However, the relative contribution and spatial routes of each transport mechanism are largely unknown. Although challenging to decipher, the details of these routes are essential for our understanding of protein transport in primary cilia, a critically affected process in many genetic diseases. By using a high-speed virtual 3D super-resolution microscopy, we have mapped the 3D spatial locations of transport routes for various cytosolic proteins in the 250-nm-wide shaft of live primary cilia with a spatiotemporal resolution of 2 ms and <16 nm. Our data reveal two spatially distinguishable transport routes for cytosolic proteins: an IFT-dependent path along the axoneme, and a passive-diffusion route in the axonemal lumen that escaped previous studies. While all cytosolic proteins tested primarily utilize the IFT path in the anterograde direction, differences are observed in the retrograde direction where IFT20 only utilizes IFT, and approximately half of KIF17 and one third of α-tubulin utilizes diffusion besides IFT.
dc.format.extent15793-
dc.language.isoen
dc.relation.haspartScientific Reports
dc.relation.isreferencedbySpringer Science and Business Media LLC
dc.rightsCC BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectAxoneme
dc.subjectCarrier Proteins
dc.subjectCilia
dc.subjectCytosol
dc.subjectDiffusion
dc.subjectGreen Fluorescent Proteins
dc.subjectKinesin
dc.subjectMice
dc.subjectMicroscopy
dc.subjectNIH 3T3 Cells
dc.subjectProbability
dc.subjectProtein Transport
dc.subjectProteins
dc.subjectTubulin
dc.titleAxonemal Lumen Dominates Cytosolic Protein Diffusion inside the Primary Cilium
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.1038/s41598-017-16103-z
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.creator.orcidYang, Weidong|0000-0002-3554-3035
dc.date.updated2021-01-22T14:44:56Z
refterms.dateFOA2021-01-22T14:45:00Z


Files in this item

Thumbnail
Name:
Axonemal Lumen Dominates Cytosolic ...
Size:
2.246Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

CC BY
Except where otherwise noted, this item's license is described as CC BY