Axonemal Lumen Dominates Cytosolic Protein Diffusion inside the Primary Cilium
Genre
Journal ArticleDate
2017-12-01Author
Luo, WRuba, A
Takao, D
Zweifel, LP
Lim, RYH
Verhey, KJ
Yang, W
Subject
AnimalsAxoneme
Carrier Proteins
Cilia
Cytosol
Diffusion
Green Fluorescent Proteins
Kinesin
Mice
Microscopy
NIH 3T3 Cells
Probability
Protein Transport
Proteins
Tubulin
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http://hdl.handle.net/20.500.12613/4851
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10.1038/s41598-017-16103-zAbstract
© 2017 The Author(s). Transport of membrane and cytosolic proteins in primary cilia is thought to depend on intraflagellar transport (IFT) and diffusion. However, the relative contribution and spatial routes of each transport mechanism are largely unknown. Although challenging to decipher, the details of these routes are essential for our understanding of protein transport in primary cilia, a critically affected process in many genetic diseases. By using a high-speed virtual 3D super-resolution microscopy, we have mapped the 3D spatial locations of transport routes for various cytosolic proteins in the 250-nm-wide shaft of live primary cilia with a spatiotemporal resolution of 2 ms and <16 nm. Our data reveal two spatially distinguishable transport routes for cytosolic proteins: an IFT-dependent path along the axoneme, and a passive-diffusion route in the axonemal lumen that escaped previous studies. While all cytosolic proteins tested primarily utilize the IFT path in the anterograde direction, differences are observed in the retrograde direction where IFT20 only utilizes IFT, and approximately half of KIF17 and one third of α-tubulin utilizes diffusion besides IFT.Citation to related work
Springer Science and Business Media LLCHas part
Scientific ReportsADA compliance
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http://dx.doi.org/10.34944/dspace/4833
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