Binary control of enzymatic cleavage of DNA origami by structural antideterminants
Genre
Journal ArticleDate
2018-01-25Author
Stopar, ACoral, L
Di Giacomo, S
Adedeji, AF
Castronovo, M
Subject
Base SequenceBinding Sites
DNA
DNA Cleavage
DNA Restriction Enzymes
Models, Molecular
Nanostructures
Nanotechnology
Nucleic Acid Conformation
Protein Binding
Protein Domains
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http://hdl.handle.net/20.500.12613/4842
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10.1093/nar/gkx1204Abstract
© The Author(s) 2017. Controlling DNA nanostructure interaction with protein is essential in developing nanodevices with programmable function, reactivity, and stability for biological and medical applications. Here, we show that the sequence-specific action of restriction endonucleases towards sharp triangular or rectangular DNA origami exhibits a novel, binary 'on/off' behaviour, as canonical recognition sites are either essentially fully reactive, or strongly resistant to enzymatic cutting. Moreover, introduction of structural defects in the sharp triangle can activate an otherwise unreactive site, with a site-to-defect distance of ∼50 nm. We argue that site reactivity is dependent upon programmable, mechanical coupling in the two-dimensional DNA origami, with specific structural elements, including DNA nicks and branches proximal to the sites that can function as negative(anti) determinants of reactivity. Empirically modelling the constraints to DNA degrees of freedom associated with each recognition site in the sharp triangle can rationalize the pattern of suppressed reactivity towards nine restriction endonucleases, in substantial agreement with the experimental results. These results provide a basis for a predictive understanding of structure-reactivity correlates of specific DNA nanostructures, which will allow a better understanding of the behaviour of nucleic acids under nanoscale confinement, as well as in the rational design of functional nanodevices based on self-assembling nucleic acids.Citation to related work
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Nucleic Acids ResearchADA compliance
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http://dx.doi.org/10.34944/dspace/4824