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dc.creatorGerstenhaber, JA
dc.creatorMarcinkiewicz, C
dc.creatorBarone, FC
dc.creatorSternberg, M
dc.creatorD’Andrea, MR
dc.creatorLelkes, PI
dc.creatorFeuerstein, GZ
dc.date.accessioned2020-12-16T19:20:40Z
dc.date.available2020-12-16T19:20:40Z
dc.date.issued2019-01-01
dc.identifier.issn1176-9114
dc.identifier.issn1178-2013
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/4583
dc.identifier.other31496697 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/4601
dc.description.abstract© 2019 Gerstenhaber et al. Background: We recently reported on long-term comprehensive biocompatibility and biodistribution study of fluorescent nanodiamond particles (NV)-Z-average 800nm (FNDP- (NV)) in rats. FNDP-(NV) primary deposition was found in the liver, yet liver function tests remained normal. Purpose: The present study aimed to gain preliminary insights on discrete localization of FNDP-(NV) in liver cells of the hepatic lobule unit and venous micro-vasculature. Kinetics of FDNP-(NV) uptake into liver cells surrogates in culture was conducted along with cell cytokinesis as markers of cells' viability. Methods: Preserved liver specimens from a pilot consisting of two animals which were stained for cytoskeletal elements (fluorescein-isothiocyanate-phalloidin) were examined for distribution of FNDP-(NV) by fluorescent microscopy (FM) and Confocal-FM (CFM) using near infra-red fluorescence (NIR). Hepatocellular carcinoma cells (HepG-2) and human umbilical vein endothelial cells (HUVEC) were cultured with FNDP-(NV) and assayed for particle uptake and location using spectrophotometric technology and microscopy. Results: HepG-2 and HUVEC displayed rapid (<30 mins) onset and concentration-dependent FNDP-(NV) internalization and formation of peri-nuclear corona. FM/CFM of liver sections revealed FNDP-(NV) presence throughout the hepatic lobules structures marked by spatial distribution, venous microvascular spaces and parenchyma and non-parenchyma cells. Conclusion: The robust presence of FNDP-(NV) throughout the hepatic lobules including those internalized within parenchyma cells and agglomerates in the liver venous microcirculation were not associated with macro or micro histopathological signs nor vascular lesions. Cells cultures indicated normal cytokinesis in cells containing FNDP-(NV) agglomerates. Liver parenchyma cells and the liver microcirculation remain agnostic to presence of FNDP-(NV) in the sinusoids or internalized in the hepatic cells.
dc.format.extent6451-6464
dc.language.isoen
dc.relation.haspartInternational Journal of Nanomedicine
dc.relation.isreferencedbyInforma UK Limited
dc.rightsCC BY-NC
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/
dc.subjectcarbon nanoparticles
dc.subjectHepG-2 cells
dc.subjectHUVEC
dc.subjectsubcellular distribution
dc.subjectcytokinesis
dc.subjectcellular uptake of nanodiamonds
dc.titleBiocompatibility studies of fluorescent diamond particles-(Nv)~800nm (part v): In vitro kinetics and in vivo localization in rat liver following long-term exposure
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.2147/IJN.S209663
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.creator.orcidLelkes, Peter|0000-0003-4954-3498
dc.creator.orcidGerstenhaber, Jonathan Arye|0000-0002-8162-7977
dc.date.updated2020-12-16T19:20:36Z
refterms.dateFOA2020-12-16T19:20:41Z


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