Show simple item record

dc.creatorStevens, RH
dc.creatorZhang, H
dc.creatorSedgley, C
dc.creatorBergman, A
dc.creatorManda, AR
dc.date.accessioned2020-12-16T18:24:00Z
dc.date.available2020-12-16T18:24:00Z
dc.date.issued2019-01-01
dc.identifier.issn2000-2297
dc.identifier.issn2000-2297
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/4565
dc.identifier.otherPMC6711143 (pmc)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/4583
dc.description.abstract© 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. Bacterial phenotypic properties are frequently influenced by the uptake of extrachromosomal genetic elements, such as plasmids and bacteriophage genomes. Such modifications can result in enhanced pathogenicity due to toxin production, increased toxin release, altered antigenicity, and resistance to antibiotics. In the case of bacteriophages, the phage genome can stably integrate into the bacterial chromosome as a prophage, to produce a lysogenic cell. Oral enterococcal strains have been isolated from subgingival plaque and the root canals of endodontically-treated teeth that have failed to heal. Previously, we isolated a bacteriophage, phage ɸEf11, induced from a lysogenic Enterococcus faecalis strain recovered from the root canal of a failed endodontic case. PCR analysis using phage ɸEf11-specific oligonucleotide primers, disclosed that lysogens containing ɸEf11 prophages were commonly found among oral E. faecalis strains, being detected in 19 of 61 (31%) strains examined. Furthermore, in comparison to an isogenic cured strain, cultures of a lysogen harboring an ɸEf11 prophage exhibited altered phenotypic characteristics, such as increased persistence at high density, enhanced biofilm formation, and resistance to a bacteriophage lytic enzyme. From these results we conclude that lysogeny is common among oral E. faecalis strains, and that it alters properties of the lysogenic cell.
dc.format.extent1643207-1643207
dc.language.isoen
dc.relation.haspartJournal of Oral Microbiology
dc.relation.isreferencedbyInforma UK Limited
dc.rightsCC BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEnterococcus faecalis
dc.subjectbacteriophage
dc.subjectlysogeny
dc.subjectphage ΦEf11
dc.subjectprophage
dc.titleThe prevalence and impact of lysogeny among oral isolates of Enterococcus faecalis
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.1080/20002297.2019.1643207
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.date.updated2020-12-16T18:23:57Z
refterms.dateFOA2020-12-16T18:24:01Z


Files in this item

Thumbnail
Name:
The prevalence and impact of ...
Size:
1.730Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

CC BY
Except where otherwise noted, this item's license is described as CC BY