Environmentally-Controlled Near Infrared Spectroscopic Imaging of Bone Water
Genre
Journal ArticleDate
2019-12-01Author
Ailavajhala, ROswald, J
Rajapakse, CS
Pleshko, N
Subject
Bone and BonesCalibration
Freeze Drying
Humans
Least-Squares Analysis
Spectroscopy, Near-Infrared
Water
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http://hdl.handle.net/20.500.12613/4540
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10.1038/s41598-019-45897-3Abstract
© 2019, The Author(s). We have designed an environmentally-controlled chamber for near infrared spectroscopic imaging (NIRSI) to monitor changes in cortical bone water content, an emerging biomarker related to bone quality assessment. The chamber is required to ensure repeatable spectroscopic measurements of tissues without the influence of atmospheric moisture. A calibration curve to predict gravimetric water content from human cadaveric cortical bone was created using NIRSI data obtained at six different lyophilization time points. Partial least squares (PLS) models successfully predicted bone water content that ranged from 0–10% (R = 0.96, p < 0.05, root mean square error of prediction (RMSEP) = 7.39%), as well as in the physiologic range of 4–10% of wet tissue weight (R = 0.87, p < 0.05, RMSEP = 14.5%). Similar results were obtained with univariate and bivariate regression models for prediction of water in the 0–10% range. Further, we identified two new NIR bone absorbances, at 6560 cm−1 and 6688 cm−1, associated with water and collagen respectively. Such data will be useful in pre-clinical studies that investigate changes in bone quality with disease, aging and with therapeutic use.Citation to related work
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http://dx.doi.org/10.34944/dspace/4522