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dc.creatorCanonico, B
dc.creatorCesarini, E
dc.creatorMontanari, M
dc.creatorDi Sario, G
dc.creatorCampana, R
dc.creatorGalluzzi, L
dc.creatorSola, F
dc.creatorGundogdu, O
dc.creatorLuchetti, F
dc.creatorDiotallevi, A
dc.creatorBaffone, W
dc.creatorGiordano, A
dc.creatorPapa, S
dc.date.accessioned2020-12-15T21:27:04Z
dc.date.available2020-12-15T21:27:04Z
dc.date.issued2020-03-02
dc.identifier.issn1661-6596
dc.identifier.issn1422-0067
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/4476
dc.identifier.other32210050 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/4494
dc.description.abstract© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The Gram-negative Campylobacter jejuni is a major cause of foodborne gastroenteritis in humans worldwide. The cytotoxic effects of Campylobacter have been mainly ascribed to the actions of the cytolethal distending toxin (CDT): it is mandatory to put in evidence risk factors for sequela development, such as reactive arthritis (ReA) and Guillain-Barré syndrome (GBS). Several researches are directed to managing symptom severity and the possible onset of sequelae. We found for the first time that rapamycin (RM) is able to largely inhibit the action of C. jejuni lysate CDT in U937 cells, and to partially avoid the activation of specific sub-lethal effects. In fact, we observed that the ability of this drug to redirect lysosomal compartment, stimulate ER-remodeling (highlighted by ER–lysosome and ER–mitochondria contacts), protect mitochondria network, and downregulate CD317/tetherin, is an important component of membrane microdomains. In particular, lysosomes are involved in the process of the reduction of intoxication, until the final step of lysosome exocytosis. Our results indicate that rapamycin confers protection against C. jejuni bacterial lysate insults to myeloid cells.
dc.format.extent2207-2207
dc.language.isoen
dc.relation.haspartInternational Journal of Molecular Sciences
dc.relation.isreferencedbyMDPI AG
dc.rightsCC BY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCampylobacter jejuni
dc.subjectrapamycin
dc.subjectER-remodeling
dc.subjectlysosome positioning and exocytosis
dc.subjectCD317
dc.subjecttetherin
dc.titleRapamycin re-directs lysosome network, stimulates er-remodeling, involving membrane CD317 and affecting exocytosis, in Campylobacter Jejuni-lysate-infected U937 cells
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.3390/ijms21062207
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.creator.orcidGiordano, Antonio|0000-0002-5959-016X
dc.date.updated2020-12-15T21:27:00Z
refterms.dateFOA2020-12-15T21:27:05Z


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