• Login
    View Item 
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of TUScholarShareCommunitiesDateAuthorsTitlesSubjectsGenresThis CollectionDateAuthorsTitlesSubjectsGenres

    My Account

    LoginRegister

    Help

    AboutPeoplePoliciesHelp for DepositorsData DepositFAQs

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    EFFECTS OF VOLUNTARY EXERCISE PRECONDITIONING ON LEFT VENTRICULAR SYSTOLIC FUNCTION AND CARDIAC AUTOPHAGY IN ANGIOTENSIN II-INDUCED HYPERTENSIVE MICE

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    TETDEDXWilson-temple-0225E-127 ...
    Size:
    3.723Mb
    Format:
    PDF
    Download
    Genre
    Thesis/Dissertation
    Date
    2016
    Author
    Wilson, Brittany Elizabeth
    Advisor
    Park, Joon Young
    Committee member
    Chen, Xiongwen
    Hudson, Matthew Bryant
    Tierney, Ryan T.
    Department
    Kinesiology
    Subject
    Kinesiology
    Health Sciences
    Autophagy
    Exercise
    Hypertension
    Permanent link to this record
    http://hdl.handle.net/20.500.12613/4043
    
    Metadata
    Show full item record
    DOI
    http://dx.doi.org/10.34944/dspace/4025
    Abstract
    Purpose/Hypothesis: Hypertension is a clinical condition with persistent elevation or raised blood pressure and accounts for approximately 9.4 million deaths every year. Exercise is one of the most effective non-pharmacological interventions and is emphasized in the current treatment guidelines for Hypertension from the World Health Organization. Currently, there is no consensus on whether autophagy is compensatory or causative in the transition from adaptive left ventricular hypertrophy to maladaptive left ventricular remodeling. It also remains unclear whether exercise preconditioning is sufficient to target autophagy for therapeutic benefits in Angiotensin II-induced hypertensive mice. The purpose of this study was to examine the effect of voluntary running exercise preconditioning on systolic function and autophagy in the heart in angiotensin II-induced hypertensive mice. Methods: Forty C57BL6/J mice were divided into 4 groups; Sedentary Sham (Sed), Sedentary Angiotensin II (SAII), Exercise Sham (Ex), Exercise Angiotensin II (ExAII). Animals in the exercise group were singly-housed and familiarized with a running wheel for 3 days prior to the study commencing. Exercise mice had access to a voluntary running wheel for 7 weeks. Ang II was infused at a constant rate using an implantable osmotic pump for the last two weeks of the experimental period. Results: Heart weight was greater in SAII, Ex and ExAII groups compared to Sed mice. Mice in Ex and ExAII groups had no significant differences in running distance or running speed. Ang II infusion caused a significantly higher thickness in the left ventricular posterior wall in diastole (LVPWd) in SAII mice which was further exacerbated in ExAII mice, creating a greater hypertrophy effect. Exercise training significantly raised systolic function as measured by ejection fraction (EF%) and fractional shortening (FS%), and exercise training prevented a significant percent reduction of EF% that was observed in SAII mice. SAII mice had significant elevations in fibrosis when compared to Sed and Ex mice, while ExAII mice had significant elevation in fibrosis when compared to Ex mice. In response to exercise training, DRP1 mRNA expression was significantly reduced, and in SAII mice protein expression of LC3II/I ratio was significantly higher which was attenuated in ExAII mice. Conclusion: Ang II resulted in a greater cardiac hypertrophy, and exercise training prevented the significant reduction in pre-post AII infusion in ejection fraction, preserving LV systolic function in ExAII mice. Ang II significantly raised the formation of autophagic machinery in SAII mice, which was attenuated in ExAII mice. This was not accompanied by a reduction in p62, indicating a possible impairment in autophagic flux in the left ventricle. Voluntary running did not alter basal autophagy, but did reduce LC3 autophagosome formation in ExAII mice and was able to attenuate the reduction in systolic function seen in SAII mice.
    ADA compliance
    For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
    Collections
    Theses and Dissertations

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Temple University Libraries | 1900 N. 13th Street | Philadelphia, PA 19122
    (215) 204-8212 | scholarshare@temple.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.