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dc.creatorKaminski, Rafal
dc.creatorChen, Yilan
dc.creatorFischer, Tracy
dc.creatorTedaldi, Ellen
dc.creatorNapoli, Alessandro
dc.creatorZhang, Yonggang
dc.creatorKarn, Jonathan
dc.creatorHu, Wenhui
dc.creatorKhalili, Kamel
dc.date.accessioned2020-09-08T15:48:57Z
dc.date.available2020-09-08T15:48:57Z
dc.date.issued2016-03-03
dc.identifier.citationKaminski, R., Chen, Y., Fischer, T. et al. Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing. Sci Rep 6, 22555 (2016). https://doi.org/10.1038/srep22555
dc.identifier.issn2045-2322
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/364
dc.identifier.urihttp://hdl.handle.net/20.500.12613/380
dc.description.abstractWe employed an RNA-guided CRISPR/Cas9 DNA editing system to precisely remove the entire HIV-1 genome spanning between 5′ and 3′ LTRs of integrated HIV-1 proviral DNA copies from latently infected human CD4+ T-cells. Comprehensive assessment of whole-genome sequencing of HIV-1 eradicated cells ruled out any off-target effects by our CRISPR/Cas9 technology that might compromise the integrity of the host genome and further showed no effect on several cell health indices including viability, cell cycle and apoptosis. Persistent co-expression of Cas9 and the specific targeting guide RNAs in HIV-1-eradicated T-cells protected them against new infection by HIV-1. Lentivirus-delivered CRISPR/Cas9 significantly diminished HIV-1 replication in infected primary CD4+ T-cell cultures and drastically reduced viral load in ex vivo culture of CD4+ T-cells obtained from HIV-1 infected patients. Thus, gene editing using CRISPR/Cas9 may provide a new therapeutic path for eliminating HIV-1 DNA from CD4+ T-cells and potentially serve as a novel and effective platform toward curing AIDS.
dc.format.extent15 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofFaculty/ Researcher Works
dc.relation.haspartScientific Reports, Vol. 6, Article 22555
dc.relation.isreferencedbyNature Research
dc.rightsAttribution CC BY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectHIV infections
dc.subjectMicrobiology
dc.titleElimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing
dc.typeText
dc.type.genreJournal article
dc.contributor.groupCenter for Neurovirology (Temple University)
dc.contributor.groupComprehensive NeuroAIDS Center (CNAC) (Temple University)
dc.description.departmentNeuroscience
dc.description.departmentMedicine
dc.relation.doihttps://doi.org/10.1038/srep22555
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeLewis Katz School of Medicine
dc.temple.creatorKaminski, Rafal
dc.temple.creatorChen, Yilan
dc.temple.creatorFischer, Tracy
dc.temple.creatorTedaldi, Ellen
dc.temple.creatorNapoli, Alessandro
dc.temple.creatorZhang, Yonggang
dc.temple.creatorHu, Wenhui
dc.temple.creatorKhalili, Kamel
refterms.dateFOA2020-09-08T15:48:57Z


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