• Login
    View Item 
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of TUScholarShareCommunitiesDateAuthorsTitlesSubjectsGenresThis CollectionDateAuthorsTitlesSubjectsGenres

    My Account

    LoginRegister

    Help

    AboutPeoplePoliciesHelp for DepositorsData DepositFAQs

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    DIFFERENTIATION OF NEURAL STEM CELL USING SMALL MOLECULES IN 2D AND 3D CULTURE SYSTEM

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    TETDEDXShi_temple_0225M_12378.pdf
    Size:
    1.419Mb
    Format:
    PDF
    Download
    Genre
    Thesis/Dissertation
    Date
    2015
    Author
    Shi, Xinglong
    Advisor
    Suh, Won H.
    Committee member
    Marcinkiewicz, Cezary
    Lazarovici, Philip
    Department
    Bioengineering
    Subject
    Engineering, Biomedical
    Alginate
    Differentiation
    Hyaluronate
    Neural Stem Cells
    Peptide Amphiphile
    Permanent link to this record
    http://hdl.handle.net/20.500.12613/3559
    
    Metadata
    Show full item record
    DOI
    http://dx.doi.org/10.34944/dspace/3541
    Abstract
    The neuronal differentiation of neural stem cells (NSCs) has received much attention due to its potential for the treatment of neurodegenerative diseases (i.e., Parkinson’s and Alzheimer’s diseases). In this regard, discovering compounds that direct differentiation of NSCs is highly required to facilitate therapeutic applications. In this study, we examined various bioactive compounds (SA1, SA2, LiCl, compound B, and DHED) to induce the neuronal differentiation of human neural stem cells (hNSCs). The study was conducted on the cells grown in three dimensional (3D) hydrogel or two dimensional (2D) environment since 3D hydrogel mimics the extracellular matrix and provides physiologically more relevant environment than 2D cell culture system. Three-dimensional (3D) hydrogel systems in this study involve polysaccharides such as alginate and hyaluronic acid. Neuronal differentiation of hNSCs was monitored in genetic level and protein level by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and immunocytochemistry (ICC), respectively. This study will show the effect of bioactive compounds on hNSCs differentiation in 2D and 3D culture systems.
    ADA compliance
    For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
    Collections
    Theses and Dissertations

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Temple University Libraries | 1900 N. 13th Street | Philadelphia, PA 19122
    (215) 204-8212 | scholarshare@temple.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.