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dc.contributor.advisorIssa, Jean-Pierre
dc.creatorGood, Charly Ryan
dc.date.accessioned2020-11-04T15:19:51Z
dc.date.available2020-11-04T15:19:51Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/20.500.12613/2937
dc.description.abstractDNA hypermethylation is known to contribute to the formation of cancer and this process has been widely studied. However, DNA hypomethylation has received far less attention and the factors controlling the balance between hypo and hypermethylation and its impact on tumorigenesis remains unclear. TET1 is a DNA demethylase that regulates DNA methylation, hydroxymethylation and gene expression. Full length TET1 (TET1FL) has a CXXC domain that binds to un-methylated CG islands (CGIs). This CXXC domain allows TET1 to protect CGIs from aberrant methylation but it also limits its ability to regulate genes outside of CGIs. This dissertation reports a novel isoform of TET1 (TET1ALT) that has a unique transcription start site from an alternate promoter in intron 2, yielding a protein with a unique translation start site. Importantly, TET1ALT lacks the CXXC domain but retains the catalytic domain. TET1ALT is repressed in ESCs but becomes activated in embryonic and adult tissues while TET1FL is ex
dc.format.extent151 pages
dc.language.isoeng
dc.publisherTemple University. Libraries
dc.relation.ispartofTheses and Dissertations
dc.rightsIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectBiology, Molecular
dc.subjectGenetics
dc.subjectCancer
dc.subjectDna Methylation
dc.subjectEpigenetics
dc.subjectTet1
dc.titleThe role of TET1 and TET1ALT in cancer
dc.typeText
dc.type.genreThesis/Dissertation
dc.contributor.committeememberEngel, Nora
dc.contributor.committeememberSapienza, Carmen
dc.contributor.committeememberBellacosa, Alfonso
dc.description.departmentBiomedical Sciences
dc.relation.doihttp://dx.doi.org/10.34944/dspace/2919
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.degreePh.D.
refterms.dateFOA2020-11-04T15:19:51Z


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