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dc.contributor.advisorTanaka, Jacqueline
dc.creatorGonzalez, Amaliris
dc.date.accessioned2020-11-04T15:19:51Z
dc.date.available2020-11-04T15:19:51Z
dc.date.issued2015
dc.identifier.other958157423
dc.identifier.urihttp://hdl.handle.net/20.500.12613/2936
dc.description.abstractLight energy is converted into an electrical signal by a set of proteins in the phototransduction cascade in photoreceptors. In this work, I focus on two critical elements of the phototransduction cascade in canine, the opsin molecules and CNG channels of cone photoreceptors. Canines are dichromats possessing two types of cone photoreceptors with different opsin molecules that detect either long and medium wavelengths (L/M) or short wavelengths (S). The L/M- and S-opsin genes were cloned from an Alaskan Malamute and used to investigate key amino acids that are responsible for tuning the spectral properties of the 11-cis retinal chromophore. Cone CNG channels are composed of CNGA3 and CNGB3 subunits. I characterized antibodies to detect cone CNG channel subunits to investigate expression of mutations in CNGB3 subunit on two canine models for achromatopsia. One model contains a missense CNGB3 mutation D262N (CNGB3m/m) and the other is a complete deletion of all exons of the CNGB3 gene (CNGB3-/-). Studies presented in this thesis show CNGB3 is expressed later in cone during retinal development compared to CNGA3. It also presents evidence for the necessity of CNGB3 in cone outer segment targeting of CNGA3.
dc.format.extent126 pages
dc.language.isoeng
dc.publisherTemple University. Libraries
dc.relation.ispartofTheses and Dissertations
dc.rightsIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectBiology
dc.subjectAchromatopsia
dc.subjectCngb3
dc.subjectCyclic Nucleotide Gated Channels
dc.subjectL/m Opsin
dc.titleEXPRESSION OF THE CNGB3 SUBUNIT IN RETINA OF ACHROMATOPSIA-AFFECTED DOGS
dc.typeText
dc.type.genreThesis/Dissertation
dc.contributor.committeememberSheffield, Joel B.
dc.contributor.committeememberHabas, Raymond
dc.contributor.committeememberYang, Weidong
dc.contributor.committeememberKomáromy, András
dc.description.departmentBiology
dc.relation.doihttp://dx.doi.org/10.34944/dspace/2918
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.degreePh.D.
refterms.dateFOA2020-11-04T15:19:51Z


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