• Login
    View Item 
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of TUScholarShareCommunitiesDateAuthorsTitlesSubjectsGenresThis CollectionDateAuthorsTitlesSubjectsGenres

    My Account

    LoginRegister

    Help

    AboutPeoplePoliciesHelp for DepositorsData DepositFAQs

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Inflammatory Effects Of Chronic Ethanol Exposure And Interactions With Cannabinoids And Spinal Cord Injury

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    TETDEDXFoss-temple-0225M-13298.pdf
    Size:
    1.597Mb
    Format:
    PDF
    Download
    Genre
    Thesis/Dissertation
    Date
    2018
    Author
    Foss, Jeffery Daniel
    Advisor
    Ward, Sara Jane
    Committee member
    Tuma, Ronald F. (Ronald Franklin)
    Eisenstein, Toby K.
    Kirby, Lynn
    Department
    Neuroscience
    Subject
    Neurosciences
    Permanent link to this record
    http://hdl.handle.net/20.500.12613/2874
    
    Metadata
    Show full item record
    DOI
    http://dx.doi.org/10.34944/dspace/2856
    Abstract
    Alcohol consumption leads to a number of disorders most notably ALD, alcoholism, and inflammation. The present study aimed to investigate the effects of a Lieber-DeCarli chronic-plus-single-binge alcohol model on animal withdrawal behaviors to 1) to determine if this model manifests any symptoms of withdrawal and 2) how severely the symptoms are expressed. To evaluate alcohol’s effect(s), a variety of behavioral assays were performed, and several of the tests exhibited signs of increased withdrawal-like behavior in ethanol exposed mice, most notably showing anxiety and convulsion-like behaviors. Inflammation is one of the main hallmarks of alcohol associated diseases and pathologies. Two of the main organs affected by alcohol induced inflammation are the brain and the liver; both of which have been shown to be structurally and physiologically disturbed by chronic alcohol consumption. Two compounds from the cannabis plant, cannabidiol and beta-caryophyllene have been isolated and shown to have anti- inflammatory properties. The potential anti-inflammatory effects of both compounds separately and in combination were assessed against ethanol exposure using a chronic- plus-single-binge alcohol model using a Lieber-DeCarli liquid diet. Immunofluorescence analysis of brain microglia and liver Kupffer cells was used to evaluate the inflammatory profile affected by alcohol and by treatment with cannabinoids. A second cohort of mice exposed to ethanol and administered CBD, BCP or both cannabinoids together was used to investigate if these compounds had effects on inflammatory cytokine concentrations or accumulation of fat in the liver. Luminex assays were used for analysis of specific cytokines in the serum and oil red o staining was used to see the potential effect on fat accumulation. Spinal cord injury results in a massive secondary inflammatory reaction that can compound on the already physically damaged tissue. Ethanol is known to induce inflammation when consumed acutely and chronically. To assess the effect of ethanol consumption on spinal cord injury we investigate pain sensitivity, motor and sensory recovery as well as the size of the lesion at the site of impact on the spinal cord. To examine motor recovery, we use a Basso Mouse Scale (BMS) for locomotion which assesses the mouse’s ability to move various parts of their hind limbs and overall coordination. Lesion size is measured using a previously characterized eriochrome cyanine with cresyl violet counterstain that elucidates damaged areas of the spinal cord in both the white and gray matter. Additionally, the glial profile was observed and quantified using a GFAP immunofluorescence stain. Interestingly, mice given ethanol had improved BMS scores when compared to control diet counterparts. Furthermore, lesion size and glial profile were also reduced in ethanol animals, showing that ethanol can have a protective, immune-suppressive effect when consumed after injury, thereby limiting secondary insult that results from SCI.
    ADA compliance
    For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
    Collections
    Theses and Dissertations

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Temple University Libraries | 1900 N. 13th Street | Philadelphia, PA 19122
    (215) 204-8212 | scholarshare@temple.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.