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dc.contributor.advisorSuh, Won H.
dc.creatorFang, Shinong
dc.date.accessioned2020-11-04T15:19:37Z
dc.date.available2020-11-04T15:19:37Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/20.500.12613/2840
dc.description.abstractThe tetra-peptide sequence alanine-valine-proline-isoleucine (AVPI) is derived from a known inhibitor of apoptosis inhibitor proteins (IAPs) called Smac (second mitochondria-derived activator of caspases). Ala-Val-Pro-Ile can be further utilized as an anti-cancer agent by inhibiting the activities of apoptosis inhibitors so caspases can trigger apoptosis of cancer cells. AVPI, however, has poorly cell-penetration property thus limiting its ability to be utilized as a therapeutic agent for cancer treatments. We conjugated the AVPI molecule to a newly developed cell-penetrating peptide (CPP) called PepB to circumvent the situation of limited cellular availability. Solid Phase Peptide Synthesis (SPPS) methods have been utilized to prepared AVPI peptide derivatives. Key characterizations involve reverse-phase high-performance liquid chromatography (RP-HPLC), mass spectrometry, optical and fluorescence microscopy.
dc.format.extent35 pages
dc.language.isoeng
dc.publisherTemple University. Libraries
dc.relation.ispartofTheses and Dissertations
dc.rightsIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectBioengineering
dc.titleTHE PREPARATION AND CHARACTERIZATION OF PRO-APOPTOTIC PEPTIDE ALA-VAL-PRO-ILE AND ITS DERIVATIVES
dc.typeText
dc.type.genreThesis/Dissertation
dc.contributor.committeememberLelkes, Peter I.
dc.contributor.committeememberPleshko, Nancy
dc.description.departmentBioengineering
dc.relation.doihttp://dx.doi.org/10.34944/dspace/2822
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.degreeM.S.
refterms.dateFOA2020-11-04T15:19:37Z


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