• Login
    View Item 
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of TUScholarShareCommunitiesDateAuthorsTitlesSubjectsGenresThis CollectionDateAuthorsTitlesSubjectsGenres

    My Account

    LoginRegister

    Help

    AboutPeoplePoliciesHelp for DepositorsData DepositFAQs

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Increased Inflammatory Gene Expression in Masseter Muscle of an Orthognathic Surgery Subject with Obstructive Sleep Apnea

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    TETDEDXConn-temple-0225M-11884.pdf
    Size:
    15.38Mb
    Format:
    PDF
    Download
    Genre
    Thesis/Dissertation
    Date
    2014
    Author
    Conn, Karen Kandel
    Advisor
    Sciote, James J.
    Committee member
    Horton, Michael J.
    Godel, Jeffrey H.
    Barton, Elisabeth R.
    Department
    Oral Biology
    Subject
    Genetics
    Dentistry
    Surgery
    Gene Expression
    Inflammation
    Masseter Muscle
    Orthodontics
    Orthognathic Surgery
    Sleep Apnea
    Permanent link to this record
    http://hdl.handle.net/20.500.12613/2715
    
    Metadata
    Show full item record
    DOI
    http://dx.doi.org/10.34944/dspace/2697
    Abstract
    Objective: Obstructive sleep apnea (OSA) is defined by recurrent breathing cessations accompanied by a collapse of the pharyngeal airway. Co-morbid conditions include obesity, cardiovascular disease, diabetes, and in some cases, retrognathia and muscle dysfunction. The latter two conditions may prompt orthognathic correction. Past investigations have shown a genetic association with OSA. Given that masseter muscle influences skeletal malocclusion and is active during OSA, we investigated whether the expression of OSA-associated genes is altered in a Class II open bite OSA subject. Methods: Eleven mandibular advancement surgery patients were classified as skeletal Class II or III and open or deep bite malocclusion, including a Class II open bite patient with OSA. Masseter muscle samples were collected at surgery and frozen. Tissue was used for gene expression analysis on Affymetrix HT2.0 microarray chips and quantitative RT-PCR. Data for ten genes associated with OSA were individually evaluated in the microarray and compared between the OSA patient and eight symmetrical malocclusion subjects. In order to corroborate these expression data, one gene of interest, tumor necrosis factor (TNF), was quantified in the ten malocclusion subjects from the microarray, an OSA subject from the microarray and one additional OSA subject by RT-PCR. Results: Among OSA-associated genes on the microarray, interleukin genes IL1B, IL1R2, IL6 and IL8 were +2.5 to +9.2 fold greater (p < 0.02) and chemokine genes CCL2, CCL3, CCL3L3, CCL4 and CXCR1 were +2.0 to +12.1 fold greater (p < 0.05). Likewise, TNF expression differed significantly in the muscle of the OSA subject (+2.2 fold greater; p < 0.001). By quantitative RT-PCR, TNF expression was significantly greater in malocclusion subjects with OSA compared to those without OSA (p = 0.0004). Conclusions: Our findings support evidence that OSA is an inflammatory disorder, which may elicit hypoxia-induced inflammatory responses believed to promote skeletal muscle dysfunction. Specifically, we report that inflammatory gene expression is significantly increased in masseter muscle in Class II open bite subjects with OSA. In turn, malocclusion may contribute to OSA, which negatively affects masseter function, resulting in exacerbation of both disorders. Because OSA is reported to associate with a polymorphism in the TNF-alpha; gene in children, future studies are needed to test for similar genetic associations in malocclusion subjects with OSA.
    ADA compliance
    For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
    Collections
    Theses and Dissertations

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Temple University Libraries | 1900 N. 13th Street | Philadelphia, PA 19122
    (215) 204-8212 | scholarshare@temple.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.