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dc.contributor.advisorSapienza, Carmen
dc.creatorSong, Sisi
dc.date.accessioned2020-11-03T15:33:45Z
dc.date.available2020-11-03T15:33:45Z
dc.date.issued2013
dc.identifier.other864885871
dc.identifier.urihttp://hdl.handle.net/20.500.12613/2436
dc.description.abstractEpidemiological data indicate that children conceived in vitro have a greater relative risk of low birth-weight, major and minor birth defects, and rare disorders involving imprinted genes, suggesting that epigenetic changes may be associated with assisted reproduction. DNA methylation and gene expression differences have been found in cord blood and placenta comparing children conceived in vitro using assisted reproductive technology (ART) and children conceived in vivo. The source of these differences (the effect of ART versus underlying infertility) has never been identified in humans. In order to determine what fraction of the DNA methylation and gene expression difference is attributable to the ART procedure and what fraction is attributable to underlying infertility, quasi-transcriptome-wide DNA methylation profiles were compared between (1) in vitro ART children of mothers who are infertile as a result of a physical impediment to fertilization (tubal blockage) or children conceived with the aid of donor oocytes as a group (tubal and donor egg group), and (2) children of parent(s) who have idiopathic infertility (infertility group). Both groups were compared to children conceived in vivo. Our data suggest strongly that many of the DNA methylation and gene expression differences observed between the in vitro and in vivo conceptions are associated with some aspect of ART procedure, rather than underlying infertility.
dc.format.extent108 pages
dc.language.isoeng
dc.publisherTemple University. Libraries
dc.relation.ispartofTheses and Dissertations
dc.rightsIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectBiology, Molecular
dc.subjectGenetics
dc.subjectArt
dc.subjectDna Methylation
dc.subjectEpigenetics
dc.subjectInfertility
dc.subjectIvf
dc.subjectPlacenta
dc.titleDNA METHYLATION DIFFERENCES BETWEEN CHILDREN CONCEIVED IN VITRO AND IN VIVO ARE ASSOCIATED WITH ART PROCEDURES
dc.typeText
dc.type.genreThesis/Dissertation
dc.contributor.committeememberLatham, Keith
dc.contributor.committeememberGraña-Amat, Xavier
dc.contributor.committeememberFisher, Jennifer O.
dc.description.departmentMolecular Biology and Genetics
dc.relation.doihttp://dx.doi.org/10.34944/dspace/2418
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.degreeM.S.
refterms.dateFOA2020-11-03T15:33:45Z


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