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dc.contributor.advisorDai, Hai-Lung
dc.creatorSharifian Gh., Mohammad
dc.date.accessioned2020-11-02T15:11:02Z
dc.date.available2020-11-02T15:11:02Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/20.500.12613/2359
dc.description.abstractUnderstanding molecular interactions at the surfaces of cellular membranes, including adsorption and transport, is of fundamental importance in both biological and pharmaceutical studies. At present, particularly with respect to small and medium size (drug-like) molecules, it is desirable to gain an understanding of the mechanisms that govern membrane adsorption and transport. To characterize drug-membrane interactions and mechanisms governing the process of molecular uptake at cellular membranes in living organisms, we need to develop effective experimental techniques to reach quantitative and time-resolved analysis of molecules at the membrane surfaces. Also, we preferably want to develop label-free optical techniques suited for single-cell and live cell analysis. Here, I discuss the nonlinear optical technique, second-harmonic light scattering (SHS), for studying molecule-membrane interactions and transport of molecules at the membrane of living cells with real-time resolution and membrane surface-specificity. Time-resolved SHS can quantify adsorption and transport of molecules, with specific nonlinear optical properties, at living organisms without imposing any mechanical stress onto the membrane. This label-free and surface-sensitive technique can even differentiate molecular transport at individual membranes within a multi-membrane cell (e.g., bacteria). In this dissertation, I present our current research and accomplishments in extending the capabilities of the SHS technique to study molecular uptake kinetics at the membranes of living cells, to monitor bacteria membrane integrity, to characterize the antibacterial mechanism-of-action of antibiotic compounds, to update the molecular mechanism of the Gram-stain protocol, to pixel-wise mapping of the membrane viscosity of the living cells, and to probe drug-induced activation of bacterial mechanosensitive channels in vitro.
dc.format.extent220 pages
dc.language.isoeng
dc.publisherTemple University. Libraries
dc.relation.ispartofTheses and Dissertations
dc.rightsIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectChemistry, Physical
dc.subjectBiophysics
dc.subjectMedicine
dc.subjectCell Membrane
dc.subjectMembrane-specific Antimicrobial Response
dc.subjectMethod Development
dc.subjectNonlinear Optical Spectroscopy and Imaging of Live Cells
dc.subjectPassive Membrane Permeation of Drug-like Molecules
dc.subjectSecond-harmonic Light Scattering
dc.titleAdsorption and Transport of Drug-Like Molecules at the Membrane of Living Cells Studied by Time-Resolved Second-Harmonic Light Scattering
dc.typeText
dc.type.genreThesis/Dissertation
dc.contributor.committeememberStanley, Robert J.
dc.contributor.committeememberWillets, Katherine A.
dc.contributor.committeememberBorguet, Eric
dc.contributor.committeememberYang, Weidong, Dr.
dc.description.departmentChemistry
dc.relation.doihttp://dx.doi.org/10.34944/dspace/2341
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.degreePh.D.
refterms.dateFOA2020-11-02T15:11:02Z


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