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dc.contributor.advisorWunder, Stephanie L.
dc.creatorSavarala, Sushma
dc.date.accessioned2020-11-02T15:10:56Z
dc.date.available2020-11-02T15:10:56Z
dc.date.issued2012
dc.identifier.other864885843
dc.identifier.urihttp://hdl.handle.net/20.500.12613/2317
dc.description.abstractThe synthesis of pyridinium cationic lipids, their counter-ion exchange, and the transfection of lipoplexes consisting of these lipids with firefly luciferase plasmid DNA (6.7 KDa), into lung, prostate and breast cancer cell lines was investigated. The transfection ability of these newly synthesized compounds was found to be twice as high as DOTAP/cholesterol and LipofectamineTM (two commercially available successful transfection agents). The compaction of the DNA onto silica (SiO2) nanoparticles was also investigated. For this purpose, it was necessary to study the stability and fusion studies of colloidal systems composed of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine), a zwitterionic lipid, and mixtures of DMPC with cationic DMTAP (1,2-dimyristoyl-3-trimethylammonium-propane).
dc.format.extent351 pages
dc.language.isoeng
dc.publisherTemple University. Libraries
dc.relation.ispartofTheses and Dissertations
dc.rightsIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectBiochemistry
dc.subjectNanoscience
dc.subjectPharmaceutical Sciences
dc.subjectCancer Therapy
dc.subjectCationic Lipids
dc.subjectColloidal Systems
dc.subjectGene Delivery
dc.subjectLipids
dc.titleControlling DNA compaction with cationic amphiphiles for efficient delivery systems-A step forward towards non-viral Gene Therapy
dc.typeText
dc.type.genreThesis/Dissertation
dc.contributor.committeememberNicholson, Allen W.
dc.contributor.committeememberVarnum, Susan A.
dc.contributor.committeememberIlies, Marc A.
dc.description.departmentChemistry
dc.relation.doihttp://dx.doi.org/10.34944/dspace/2299
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.degreePh.D.
refterms.dateFOA2020-11-02T15:10:56Z


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