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dc.contributor.advisorPatil, Chetan Appasaheb
dc.creatorRashvand, Shahriar Cyrus
dc.date.accessioned2020-11-02T14:46:48Z
dc.date.available2020-11-02T14:46:48Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/20.500.12613/2213
dc.description.abstractAdipose tissue is a type of connective tissue whose purpose was once thought to be limited to fat storage but is now understood to be a key factor in the pathogenesis of different metabolic diseases, including obesity and type-II diabetes. Adipose tissue consists largely of adipocytes, cells responsible for fat and releasing energy in form of lipids. Different classes of fatty acids, such as saturated and unsaturated have different biological effects on adipocytes. Lipid droplets are the primary organelles in adipocytes that store these fatty acids in form of lipids, and the development of engineered adipose tissues would benefit from improved techniques for analysis of lipid droplet composition, distributions, and dynamics based as a function of fatty acid saturation. Conventional microscopic techniques, such as fluorescence microscopy, provides excellent selectivity of lipid-based structures inside adipose tissue cellular structures based on staining with compound dyes. However, fluorescence staining limits multiplex imaging, and requires time consuming steps in preparing the samples for imaging. Therefore, developing a label-free, high resolution imaging platform with sensitivity to lipid composition could enable analysis of structural and compositional differentiation of lipid droplets within adipocytes during differentiation could give valuable insights into the importance of lipid droplets role in metabolism. As an important step towards achieving this goal, a femtosecond based CARS microscopy imaging platform has been developed to perform in vitro, label-free, imaging of fatty acid composition within engineered adipose tissues.
dc.format.extent78 pages
dc.language.isoeng
dc.publisherTemple University. Libraries
dc.relation.ispartofTheses and Dissertations
dc.rightsIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectBioengineering
dc.subjectCars Microscopy
dc.subjectEngineered Adipose Tissue
dc.subjectFemtosecond
dc.subjectLipid Droplets
dc.titleFemtosecond CARS Microscopy to characterize lipid droplets in Engineered Adipose Tissue
dc.typeText
dc.type.genreThesis/Dissertation
dc.contributor.committeememberBellas, Evangelia
dc.contributor.committeememberGligorijevic, Bojana
dc.description.departmentBioengineering
dc.relation.doihttp://dx.doi.org/10.34944/dspace/2195
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.degreeM.S.
refterms.dateFOA2020-11-02T14:46:48Z


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