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dc.contributor.advisorFoster, William J.
dc.creatorMacPherson, Meoghan E.
dc.date.accessioned2020-10-27T15:14:19Z
dc.date.available2020-10-27T15:14:19Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/20.500.12613/1802
dc.description.abstractCentral to ocular health is the vitreous body, a complex, gelatinous tissue filling the space between the lens and retina. It is a natural polymeric hydrogel whose delicate architecture of collagen and hyaluronic acid loses its mechanical structure under the influence of degeneration or destruction, leaving the retina vulnerable to injury and disease. Since World War II, combat ocular trauma has increased six-fold while the population of aging veterans continues to grow in tandem. Compared to injuries in the civilian sector, injuries in theaters of combat operations are sustained in dirty, dusty, high-stress environments under hostile fire. These penetrating and perforating ocular injuries have predicable consequences, cascading into scarring on or under the retina (known as proliferative vitreoretinopathy or PVR). The growing population of aging veterans also faces a multitude of vitreous-related and vision-threatening pathologies. Current standards of care call for the removal and replacement of the vitreous, but contemporary substitutes are ill suited for long-term use. As such, there is critical need for development of a successful, long-term vitreous substitute. A biomimetic in situ forming hydrogel has been developed that utilizes a reversible disulfide cross-linker, enabling easy injection into the vitreous cavity. Recently, a copolymer has been introduced with this new formulation that possesses a unique comb-like structure whose characteristic bristles inhibit protein adsorption and cellular adherence, perfectly suited for the inhibition of PVR. The objective of this dissertation was to evaluate select formulations of this unique in situ forming hydrogel as potential vitreous substitutes. This was accomplished through rigorous in vivo rabbit modeled testing of long-term biocompatibility and bioperformance utilizing electroretinography, clinical examination, and histopathological assessment. We hypothesized that the in situ forming hydrogels would serve as a substantial improvement over the current gold standard, silicone oil, in terms of biocompatibility and the ability to inhibit PVR.
dc.format.extent104 pages
dc.language.isoeng
dc.publisherTemple University. Libraries
dc.relation.ispartofTheses and Dissertations
dc.rightsIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectEngineering, Biomedical
dc.subjectOphthalmology
dc.subjectDutch-belted
dc.subjectElectroretinogram
dc.subjectIn Situ
dc.subjectPhosphocholine
dc.subjectProliferative Vitreoretinopathy
dc.subjectVitreous
dc.titleFrom Bench to Battlefield: An Evaluation of in situ Forming Hydrogels as Vitreous Substitutes for Military and Combat Veterans
dc.typeText
dc.type.genreThesis/Dissertation
dc.contributor.committeememberLemay, Michel A.
dc.contributor.committeememberLelkes, Peter I.
dc.contributor.committeememberBrodie, Scott E.
dc.description.departmentBioengineering
dc.relation.doihttp://dx.doi.org/10.34944/dspace/1784
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.degreePh.D.
refterms.dateFOA2020-10-27T15:14:19Z


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