• Login
    View Item 
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of TUScholarShareCommunitiesDateAuthorsTitlesSubjectsGenresThis CollectionDateAuthorsTitlesSubjectsGenres

    My Account

    LoginRegister

    Help

    AboutPeoplePoliciesHelp for DepositorsData DepositFAQs

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    ROLE OF INTERLEUKIN-19 AND ALLOGRAFT INFLAMMATORY FACTOR-1 IN ENDOTHELIAL CELL PROLIFERATION, ACTIVATION, MIGRATION AND ANGIOGENIC POTENTIAL

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    JAIN_temple_0225E_10013.pdf
    Size:
    11.11Mb
    Format:
    PDF
    Download
    Genre
    Thesis/Dissertation
    Date
    2009
    Author
    JAIN,SURBHI
    Advisor
    Tsygankov, Alexander Y.
    Committee member
    Autieri, Michael V.
    Monestier, Marc
    Ganea, Doina
    Rizzo, Victor
    Department
    Microbiology and Immunology
    Subject
    Biology, Microbiology
    Health Sciences, Immunology
    Allograft Inflammatory Factor-1
    Angiogenesis
    Endothelial Cell
    Interleukin-19
    Migration
    Proliferation
    Permanent link to this record
    http://hdl.handle.net/20.500.12613/1522
    
    Metadata
    Show full item record
    DOI
    http://dx.doi.org/10.34944/dspace/1504
    Abstract
    Angiogenesis is an important process in maintaining normal physiology as well as in the pathology of many diseases. Angiogenesis based therapies have the potential to have a phenomenal impact on a diseases affecting more than one billion people worldwide, including all cancers, cardiovascular disease, blindness, arthritis, complications of AIDS, diabetes, Alzheimer's disease, and more than 70 other major health conditions affecting children and adults, in developed and developing nations. In this study, we investigate the role of Interleukin-19 (IL-19) and Allograft inflammatory factor-1 (AIF-1) in endothelial cells (EC) proliferation, migration, activation and angiogenic potential. IL-19 is a recently described member of the IL-10 family of anti-inflammatory cytokines. Nothing has been reported on the expression or mechanism(s) of IL-19 effects in endothelial cells. We have found that IL-19 is expressed in aortic endothelium, and can be induced in cultured EC by serum and inflammatory cytokine challenge. IL-19 is chemotactic for EC, and promotes cell spreading, migration, and wound healing. IL-19 pretreatment also enhances the migration of EC to Vascular endothelial growth factor (VEGF). IL-19 activates the signaling proteins STAT3, p44/42 MAPK, and Rac1, and induces expression of Matrix Metalloproteinase-2, which is obligate for EC migration. IL-19 promotes tube -like structure formation on Matrigel by Human Umbilical Vein EC (HUVEC), and promotes microvessel formation in mouse aortic ring assay. Taken together, these data suggest that IL-19 is a chemotactic cytokine for, and promotes the proliferation, migration, activation and angiogenic potential of endothelial cells. AIF-1 is a cytoplasmic, calcium-binding, inflammation-responsive scaffold protein that has been implicated in the regulation of inflammation. The expression and function of AIF-1 in EC is uncharacterized. AIF-1 expression co-localized with CD31-positive endothelial cells in neointima of inflamed human arteries, but not normal arteries. AIF-1 is detected at low levels in unstimulated EC, but expression can be increased in response to serum and soluble factors. Stable transfection of AIF-1 siRNA in EC reduced AIF-1 protein expression by 73%, and significantly reduced EC proliferation and migration. Rescue of AIF-1 expression restored both proliferation and migration of siRNA expressing ECs, and AIF-1 over expression enhanced both of these activities, suggesting a strong association between AIF-1 expression and EC activation. Activation of MAPK p44/42 and PAK1 was significantly reduced in siRNA ECs challenged with inflammatory stimuli. Reduction of AIF-1 expression did not decrease EC tube-like structure or microvessel formation from aortic rings, but over-expression of AIF-1 did significantly increase the number and complexity of these structures. These data indicate that AIF-1 expression plays an important role in proliferation, migration, signal transduction, and may have a role in angiogenesis.
    ADA compliance
    For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
    Collections
    Theses and Dissertations

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Temple University Libraries | 1900 N. 13th Street | Philadelphia, PA 19122
    (215) 204-8212 | scholarshare@temple.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.