Antibiotic Movement through Heterogeneous Biofilms
dc.contributor.advisor | Queisser, Gillian | |
dc.creator | Henry, Brandi | |
dc.date.accessioned | 2024-09-12T19:35:08Z | |
dc.date.available | 2024-09-12T19:35:08Z | |
dc.date.issued | 2024-08 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12613/10718 | |
dc.description.abstract | Biofilms are communities of microorganisms that can form in the human microbiome and on medical implants among other locations. These communities provide greater protection for their member cells resulting in an increase in resistance to antibiotic treatment and persistent infections. There are several factors that may contribute to antibiotic resistance of biofilms. These studies were done concurrently with biological experiments to test the hypothesis that dense, rigid structures within the biofilm may be an additional mechanism for protection from antibiotics. A computational tool and workflow was developed to analyze bead movement for the characterization of biofilm biomaterial properties including rigidity. With this tool, the analysis revealed that the amyloid, curli, confers rigidity in biofilms, thereby restricting bead movement. Greater movement of the beads is seen in biofilms lacking curli and biofilms that produced complex heterogeneous rigid structures. A new model was also developed that uses microscopy imaging data to simulate diffusion-reaction of antibiotics within heterogeneous biofilms. This model was used to investigate the effect of the dense, rigid structures on antibiotic treatment through test simulations and simulations using biological imaging data. These studies reveal various properties about the dense, rigid structures that confer protection. | |
dc.format.extent | 222 pages | |
dc.language.iso | eng | |
dc.publisher | Temple University. Libraries | |
dc.relation.ispartof | Theses and Dissertations | |
dc.rights | IN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available. | |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | |
dc.subject | Applied mathematics | |
dc.subject | Antibiotic resistance | |
dc.subject | Biofilms | |
dc.subject | Mathematical modeling | |
dc.subject | Numerical simulations | |
dc.subject | Partial differential equations | |
dc.title | Antibiotic Movement through Heterogeneous Biofilms | |
dc.type | Text | |
dc.type.genre | Thesis/Dissertation | |
dc.contributor.committeemember | Seibold, Benjamin | |
dc.contributor.committeemember | Klapper, Isaac | |
dc.contributor.committeemember | Buttaro, Bettina A. | |
dc.description.department | Mathematics | |
dc.relation.doi | http://dx.doi.org/10.34944/dspace/10680 | |
dc.ada.note | For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu | |
dc.description.degree | Ph.D. | |
dc.identifier.proqst | 15885 | |
dc.date.updated | 2024-08-30T19:08:13Z | |
refterms.dateFOA | 2024-09-12T19:35:09Z | |
dc.identifier.filename | Henry_temple_0225E_15885.pdf |