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dc.contributor.advisorQueisser, Gillian
dc.creatorHenry, Brandi
dc.date.accessioned2024-09-12T19:35:08Z
dc.date.available2024-09-12T19:35:08Z
dc.date.issued2024-08
dc.identifier.urihttp://hdl.handle.net/20.500.12613/10718
dc.description.abstractBiofilms are communities of microorganisms that can form in the human microbiome and on medical implants among other locations. These communities provide greater protection for their member cells resulting in an increase in resistance to antibiotic treatment and persistent infections. There are several factors that may contribute to antibiotic resistance of biofilms. These studies were done concurrently with biological experiments to test the hypothesis that dense, rigid structures within the biofilm may be an additional mechanism for protection from antibiotics. A computational tool and workflow was developed to analyze bead movement for the characterization of biofilm biomaterial properties including rigidity. With this tool, the analysis revealed that the amyloid, curli, confers rigidity in biofilms, thereby restricting bead movement. Greater movement of the beads is seen in biofilms lacking curli and biofilms that produced complex heterogeneous rigid structures. A new model was also developed that uses microscopy imaging data to simulate diffusion-reaction of antibiotics within heterogeneous biofilms. This model was used to investigate the effect of the dense, rigid structures on antibiotic treatment through test simulations and simulations using biological imaging data. These studies reveal various properties about the dense, rigid structures that confer protection.
dc.format.extent222 pages
dc.language.isoeng
dc.publisherTemple University. Libraries
dc.relation.ispartofTheses and Dissertations
dc.rightsIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectApplied mathematics
dc.subjectAntibiotic resistance
dc.subjectBiofilms
dc.subjectMathematical modeling
dc.subjectNumerical simulations
dc.subjectPartial differential equations
dc.titleAntibiotic Movement through Heterogeneous Biofilms
dc.typeText
dc.type.genreThesis/Dissertation
dc.contributor.committeememberSeibold, Benjamin
dc.contributor.committeememberKlapper, Isaac
dc.contributor.committeememberButtaro, Bettina A.
dc.description.departmentMathematics
dc.relation.doihttp://dx.doi.org/10.34944/dspace/10680
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.degreePh.D.
dc.identifier.proqst15885
dc.date.updated2024-08-30T19:08:13Z
refterms.dateFOA2024-09-12T19:35:09Z
dc.identifier.filenameHenry_temple_0225E_15885.pdf


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