• Amniote phylogeny and the position of turtles

      Hedges, SB (2012-07-27)
      The position of turtles among amniotes remains in dispute, with morphological and molecular comparisons giving different results. Morphological analyses align turtles with either lizards and their relatives, or at the base of the reptile tree, whereas molecular analyses, including a recent study by Chiari et al. in BMC Biology, place turtles with birds and crocodilians. Molecular studies have not wavered as the numbers of genes and species have increased, but morphologists have been reluctant to embrace the molecular tree.Please see Research article www.biomedcentral.com/1741-7007/10/65. © 2012 Hedges; licensee BioMed Central Ltd.
    • Fish mutant, where is thy phenotype?

      Balciunas, D; Balciunas, Darius|0000-0003-1938-3243 (2018-02-01)
    • Functional genetics in the post-genomics era: building a better roadmap in Drosophila.

      Kulathinal, RJ; Kulathinal, Rob|0000-0003-1907-2744 (2013-01-01)
      In this commentary, Rob Kulathinal describes two papers from the Perrimon laboratory, each describing a new online resource that can assist geneticists with the design of their RNAi experiments. Hu et al.'s "UP-TORR: online tool for accurate and up-to-date annotation of RNAi reagents" and "FlyPrimerBank: An online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents" are published, respectively, in this month's issue of GENETICS and G3: Genes|Genomes|Genetics.
    • General parenting styles and children's obesity risk: Changing focus

      Larsen, JK; Sleddens, EFC; Vink, JM; Fisher, JO; Kremers, SPJ (2018-11-06)
    • Invadopodia in context

      Bergman, A; Condeelis, JS; Gligorijevic, B; Gligorijevic, Bojana|0000-0001-9071-7467 (2014-05-01)
      © 2014 Landes Bioscience. Invadopodia are dynamic protrusions in motile tumor cells whose function is to degrade extracellular matrix so that cells can enter into new environments. Invadopodia are specifically identified by microscopy as proteolytic invasive protrusions containing TKS5 and cortactin. The increasing complexity in models for the study of invadopodia, including engineered 3D environments, explants, or animal models in vivo, entails a higher level of microenvironment complexity as well as cancer cell heterogeneity. Such experimental setups are rich in information and offer the possibility of contextualizing invadopodia and other motility-related structures. That is, they hold the promise of revealing more realistic microenvironmental conditions under which the invadopodium assembles and functions or in which tumor cells switch to a different cellular phenotype (focal adhesion, lamellipodia, proliferation, and apoptosis). For such an effort, we need a systemic approach to microscopy, which will integrate information from multiple modalities. While the individual technologies needed to achieve this are mostly available, data integration and standardization is not a trivial process. In a systems microscopy approach, microscopy is used to extract information on cell phenotypes and the microenvironment while -omics technologies assess profiles of cancer cell and microenvironment genetic, transcription, translation, and protein makeups. Data are classified and linked via in silico modeling (including statistical and mathematical models and bioinformatics). Computational considerations create predictions to be validated experimentally by perturbing the system through use of genetic manipulations and molecular biology. With such a holistic approach, a deeper understanding of function of invadopodia in vivo will be reached, opening the potential for personalized diagnostics and therapies.
    • Law and neuroscience: Recommendations submitted to the president's bioethics commission

      Jones, OD; Bonnie, RJ; Casey, BJ; Davis, A; Faigman, DL; Hoffman, M; Montague, R; Morse, SJ; Raichle, ME; Richeson, JA; Scott, E; Steinberg, L; Taylor-Thompson, K; Wagner, A; Yaffe, G (2014-09-01)