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A Photo-crosslinkable Soy-derived Bioink for 3D Bioprinting
Schwab, Kyle
Schwab, Kyle
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Thesis/Dissertation
Date
2021
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Bioengineering
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http://dx.doi.org/10.34944/dspace/6806
Abstract
Soy protein isolate (SPI) has become increasingly attractive for tissue engineering purposes because of its abundance in nature (a plant-derived protein), ease of isolation and processing, customizable biodegradability, inexpensive cost, and minimal immunogenicity. Combining SPI with methacrylic anhydride to form soy-methacryloyl (SoyMA) makes it possible to develop a semi-synthetic bioink that can recapitulate in-vivo tissue constructs when extruded by a three-dimensional (3D) bioprinter. Bioinks offer an ideal biological microenvironment for cells and can be photo-crosslinked easily, ensuring cell encapsulation and form fidelity. The long-term goal of this research is to demonstrate that SoyMA bioinks can be synthesized to form a 3D cell culture material with a particular degree of functionalization (DoF). Specifically, I propose to develop SoyMA bioinks that can be used to fabricate scaffolds mimicking the microenvironment of spinal cord tissue using neuronal progenitor cells (pheochromocytoma (PC12) cells) and endothelial cells (EC). Using 3D bioprinting, we will test the ability of these scaffolds to promote cell adhesion, directed spreading, and proliferation. TO control the mechanical properties of SoyMA, we will parametrically vary polymerization conditions such as concentration, DoF, and photo-crosslinking. We will also evaluate and compare cell viability and morphology of cells grown in various stiffnesses of SoyMA scaffolds. Taken together, we will demonstrate how SoyMA bioinks, coupled with 3D bioprinting, can be used to fabricate dynamic and tunable tissue scaffolds.
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