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Analysis of Interactions Between Candida Albicans and Enterococcus Faecalis in an Iron Rich Environment
Revelt, Luke
Revelt, Luke
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Date
2023-08
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Oral Biology
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http://dx.doi.org/10.34944/dspace/8873
Abstract
Objective: Failed root canals have been associated with a variety of microorganisms, such as Entercoccus faecalis (E. faecalis) and Candida albicans (C. albicans). The goal
of the present study is to better understand the interactions between E. faecalis and C.
albicans. Specifically, the cellular morphology of these interactions and the effect on cell
aggregation and biofilm formation. Since much of C. albicans’ adhesion mechanisms are
regulated by high iron levels, this study also seeks to analyze the role of iron in the
interactions between C. albicans and E. faecalis.
Methods: Individual cultures of E. faecalis and C. albicans as well as co-cultures of both were prepared in 1:1 mixture of brain heart infusion and RPMI media in high and low
iron conditions. Growth curves were created for each culture. Biofilm formation of
cultures was measured through crystal violet analysis. Sedimentation assay at OD600 was
used to measure cellular aggregation. Cells were observed under microscope to view
cellular interactions.
Results: Regardless of iron levels, growth of C. albicans was reduced when grown in co-culture with E. faecalis (p < 0.0001). Biofilm formation was reduced in cocultures of E.
faecalis and C. albicans under high iron conditions, OD600=2.13, compared low iron
conditions, OD600=3.60 (p < 0.0001). Microscopy showed a lack of aggregation of E.
faecalis along C. albicans hyphae.
Conclusion: E. faecalis and C. albicans show an antagonistic relationship that restricts normal biofilm formation and growth in high iron conditions.
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