Loading...
Invadopodia enable cooperative invasion and metastasis of breast cancer cells
Belova, Elizaveta Bayarmagnai, Battuya
Belova, Elizaveta
Bayarmagnai, Battuya
Citations
Altmetric:
Genre
Journal article
Date
2022-08-01
Advisor
Committee member
Group
Department
Bioengineering
Subject
Permanent link to this record
Collections
Research Projects
Organizational Units
Journal Issue
DOI
https://doi.org/10.1038/s42003-022-03642-z
Abstract
Invasive and non-invasive cancer cells can invade together during cooperative invasion. However, the events leading to it, role of the epithelial-mesenchymal transition and the consequences this may have on metastasis are unknown. In this study, we demonstrate that the isogenic 4T1 and 67NR breast cancer cells sort from each other in 3D spheroids, followed by cooperative invasion. By time-lapse microscopy, we show that the invasive 4T1 cells move more persistently compared to non-invasive 67NR, sorting and accumulating at the spheroid-matrix interface, a process dependent on cell-matrix adhesions and independent from E-cadherin cell-cell adhesions. Elimination of invadopodia in 4T1 cells blocks invasion, demonstrating that invadopodia requirement is limited to leader cells. Importantly, we demonstrate that cells with and without invadopodia can also engage in cooperative metastasis in preclinical mouse models. Altogether, our results suggest that a small number of cells with invadopodia can drive the metastasis of heterogeneous cell clusters.
Description
Citation
Perrin, L., Belova, E., Bayarmagnai, B. et al. Invadopodia enable cooperative invasion and metastasis of breast cancer cells. Commun Biol 5, 758 (2022). https://doi.org/10.1038/s42003-022-03642-z
Citation to related work
Nature Research
Has part
Communications Biology, Vol. 5
ADA compliance
For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu