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Thesis/Dissertation
Date
2021
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Biomedical Sciences
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http://dx.doi.org/10.34944/dspace/7209
Abstract
G-quadruplexes, a type of nucleic acid secondary structure consisting largely of folded quartets of guanines, appear to play a regulatory role in the human genome. Heme has been shown to interact with G-quadruplexes. The ChIP-Seq-like Heme-Seq assay was developed to identify heme binding G-quadruplex loci. Using Heme-Seq, 3 primary heme binding loci and 4 secondary minor heme binding loci were identified on six chromosomes. Two of the primary heme binding loci were found at the centromeric boundaries of the long arms of metacentric chromosomes with the majority of reads from the primary heme binding loci consisting primarly of Human Satellite II (HSATII) nucleotide repeat sequences. Numerous putative G-quadruplex forming sequences were found in the heme-binding locus on Chromosome 2. Comparison of Heme-Seq results with available data from a G-quadruplex ChIP-Seq study in live cells, revealed that the regions which exhibited binding at the three peaks from the Heme-Seq data also showed binding coverage in the CHIP-Seq data. In addition to the known association with G-quadruplexes, heme also appears to bind to HSATII repeats,. The biological role and importance of this binding is not known.
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