Thumbnail Image
Item

Internal epitope tagging informed by relative lack of sequence conservation

Burg, L
; 
Zhang, K
; 
Bonawitz, T
; 
Grajevskaja, V
; 
Bellipanni, G
; 
Waring, R
; 
Balciunas, D
Citations
Altmetric:
Genre
Journal Article
Date
2016-11-28
Advisor
Committee member
Group
Department
Subject
Amino Acid Sequence
 Animals
 Antibodies
 Conserved Sequence
 Epitopes
 Proteins
 Sequence Alignment
 Zebrafish
Permanent link to this record
http://hdl.handle.net/20.500.12613/4993
Collections
Faculty/Researcher Works
Show all metadata
Files
Thumbnail Image
Internal epitope tagging informed by relative lack of sequence conservation.pdf
Adobe PDF, 3.17 MB
Research Projects
Organizational Units
Journal Issue
DOI
10.1038/srep36986
Abstract
Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function.
Description
Citation
Citation to related work
Springer Science and Business Media LLC
Has part
Scientific Reports
ADA compliance
For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
Embedded videos