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Global transcriptional response of oral squamous cell carcinoma cell lines to health-associated oral bacteria - an in vitro study
Chitrala, Kumaraswamy naidu
Chitrala, Kumaraswamy naidu
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2022-05-16
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http://dx.doi.org/10.1080/20002297.2022.2073866
Abstract
Background: We have recently demonstrated that health-associated oral bacteria Streptococcus mitis, Neisseria flavescens, and Haemophilus parainfluenzae induce cytotoxicity in oral squamous cell carcinoma (OSCC) cell lines and downregulate CD36, a cancer-assocaited gene. Aim: To explore the effect of these three species on global transcriptome of OSCC cell lines. Methods: Gene expression of cell lines CAL27, SCC4 and SCC25 cocultured with the test species was assessed with Clariom-S Human microarray. Porphyromonas gingivalis was included as a pathogenic control. Data were analyzed using Ingenuity Pathway Analysis. Results: The results differed by species and cell line. Overall, the transcriptional changes by S. mitis were predominantly anti-cancer including inhibition of HOTAIR regulatory pathway, JAK/Stat signaling, cyclins/cyclin-dependent kinases, and endothelin1 signaling. H. parainfluenzae and N. flavescens resulted in a mix of pro- and anti-cancer responses including activation of acute phase response, pro-inflammatory interleukins signaling, TREM-1 signaling, and tumor microenvironment pathway; but downregulation of cell cycle by inhibition of cyclins and cyclin-dependent kinases. P. gingivalis had a predominantly pro-cancer effect limited to SCC4, including upregulation of inflammatory pathways, phospholipases and PI3K signaling. Conclusion: These findings provide a new insight into the role of commensal oral bacteria in OSCC. Animal studies are required to further explore them.
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Divyashri Baraniya, Kumaraswamy Naidu Chitrala & Nezar Noor Al-Hebshi (2022) Global transcriptional response of oral squamous cell carcinoma cell lines to health-associated oral bacteria - an in vitro study, Journal of Oral Microbiology, 14:1, DOI: 10.1080/20002297.2022.2073866
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Taylor and Francis Group
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Journal of Oral Microbiology, Vol. 14, Iss. 1
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