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Analysis of RNA conformation in endogenously assembled RNPs by icSHAPE
Chang, Howard Y. Artandi, Steven E.
Chang, Howard Y.
Artandi, Steven E.
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Journal article
Date
2021-04-28
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Committee member
Department
Cancer and Cellular Biology
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https://doi.org/10.1016/j.xpro.2021.100477
Abstract
The majority of the mammalian genome is transcribed into non-coding RNAs, many of which co-evolve with RNA-binding proteins (RBPs) to function as biochemically defined and tractable ribonucleoproteins (RNPs). Here, we applied icSHAPE- a robust and versatile RNA structural probing pipeline- to endogenous RNPs purified from nuclei, providing base-resolution structural rationale for RNP activity and subcellular localization. Combining with genetic and biochemical reconstitutions, structural and functional alternations can be directly attributed to a given RBP without ambiguity.
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Citation
Chen L, Chang HY, Artandi SE. Analysis of RNA conformation in endogenously assembled RNPs by icSHAPE. STAR Protocols. 2021 Jun 18;2(2). doi:10.1016/j.xpro.2021.100477.
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Cell Press
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STAR Protocols, Vol. 2, Iss. 2
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