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Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution

Mudumbi, KC
; 
Schirmer, EC
; 
Yang, W
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Journal Article
Date
2016-08-25
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Subject
Biological Transport
 Endoplasmic Reticulum
 Feasibility Studies
 Fluorescence Recovery After Photobleaching
 HeLa Cells
 Humans
 Membrane Proteins
 Microscopy, Confocal
 Microscopy, Fluorescence
 Nuclear Envelope
 Single Molecule Imaging
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http://hdl.handle.net/20.500.12613/5031
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Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution.pdf
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10.1038/ncomms12562
Abstract
© The Author(s) 2016. The normal distribution of nuclear envelope transmembrane proteins (NETs) is disrupted in several human diseases. NETs are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM). Quantitative determination of the distribution of NETs on the ONM and INM is limited in available approaches, which moreover provide no information about translocation rates in the two membranes. Here we demonstrate a single-point single-molecule FRAP microscopy technique that enables determination of distribution and translocation rates for NETs in vivo.
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Springer Science and Business Media LLC
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Nature Communications
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