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Plasmodium falciparum K76T pfcrt gene mutations and parasite population structure, Haiti, 2006–2009
Charles, M Das, S Daniels, R Kirkman, L Delva, GG Destine, R Escalante, A Villegas, L Daniels, NM Shigyo, K
Charles, M
Das, S
Daniels, R
Kirkman, L
Delva, GG
Destine, R
Escalante, A
Villegas, L
Daniels, NM
Shigyo, K
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Genre
Journal Article
Date
2016-05-01
Advisor
Committee member
Group
Department
Subject
CQR
CQS
Haiti
Hispaniola
Jeremie
K76T pfcrt
Les Cayes
Plasmodium falciparum
chloroquine
gene mutation
haplotype
malaria
parasites
vector-borne infections
vectorborne
DNA Barcoding, Taxonomic
Geography
Haiti
History, 21st Century
Humans
Malaria, Falciparum
Membrane Transport Proteins
Mutation
Phylogeography
Plasmodium falciparum
Protozoan Proteins
Sequence Analysis, DNA
CQS
Haiti
Hispaniola
Jeremie
K76T pfcrt
Les Cayes
Plasmodium falciparum
chloroquine
gene mutation
haplotype
malaria
parasites
vector-borne infections
vectorborne
DNA Barcoding, Taxonomic
Geography
Haiti
History, 21st Century
Humans
Malaria, Falciparum
Membrane Transport Proteins
Mutation
Phylogeography
Plasmodium falciparum
Protozoan Proteins
Sequence Analysis, DNA
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DOI
10.3201/eid2205.150359
Abstract
© 2016, Centers for Disease Control and Prevention (CDC). All rights reserved. Hispaniola is the only Caribbean island to which Plasmodium falciparum malaria remains endemic. Resistance to the antimalarial drug chloroquine has rarely been reported in Haiti, which is located on Hispaniola, but the K76T pfcrt (P. falciparum chloroquine resistance transporter) gene mutation that confers chloroquine resistance has been detected intermittently. We analyzed 901 patient samples collected during 2006–2009 and found 2 samples showed possible mixed parasite infections of genetically chloroquine-resistant and -sensitive parasites. Direct sequencing of the pfcrt resistance locus and single-nucleotide polymorphism barcoding did not definitively identify a resistant population, suggesting that sustained propagation of chloroquine-resistant parasites was not occurring in Haiti during the study period. Comparison of parasites from Haiti with those from Colombia, Panama, and Venezuela reveals a geographically distinct population with highly related parasites. Our findings indicate low genetic diversity in the parasite population and low levels of chloroquine resistance in Haiti, raising the possibility that reported cases may be of exogenous origin.
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Centers for Disease Control and Prevention (CDC)
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Emerging Infectious Diseases
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