Loading...
Recent Zoonotic Spillover and Tropism Shift of a Canine Coronavirus Is Associated with Relaxed Selection and Putative Loss of Function in NTD Subdomain of Spike Protein
Martin, Darren P. Ceres, Kristina Whittaker, Gary R. Millet, Jean K. Goodman, Laura B. Stanhope, Michael J.
Martin, Darren P.
Ceres, Kristina
Whittaker, Gary R.
Millet, Jean K.
Goodman, Laura B.
Stanhope, Michael J.
Citations
Altmetric:
Genre
Journal article
Date
2022-04-21
Advisor
Committee member
Department
Biology
Permanent link to this record
Collections
Research Projects
Organizational Units
Journal Issue
DOI
https://doi.org/10.3390/v14050853
Abstract
A canine coronavirus (CCoV) has now been reported from two independent human samples from Malaysia (respiratory, collected in 2017–2018; CCoV-HuPn-2018) and Haiti (urine, collected in 2017); these two viruses were nearly genetically identical. In an effort to identify any novel adaptations associated with this apparent shift in tropism we carried out detailed evolutionary analyses of the spike gene of this virus in the context of related Alphacoronavirus 1 species. The spike 0-domain retains homology to CCoV2b (enteric infections) and Transmissible Gastroenteritis Virus (TGEV; enteric and respiratory). This domain is subject to relaxed selection pressure and an increased rate of molecular evolution. It contains unique amino acid substitutions, including within a region important for sialic acid binding and pathogenesis in TGEV. Overall, the spike gene is extensively recombinant, with a feline coronavirus type II strain serving a prominent role in the recombinant history of the virus. Molecular divergence time for a segment of the gene where temporal signal could be determined, was estimated at around 60 years ago. We hypothesize that the virus had an enteric origin, but that it may be losing that particular tropism, possibly because of mutations in the sialic acid binding region of the spike 0-domain.
Description
Citation
Zehr, Jordan D., Sergei L. Kosakovsky Pond, Darren P. Martin, Kristina Ceres, Gary R. Whittaker, Jean K. Millet, Laura B. Goodman, and Michael J. Stanhope. 2022. "Recent Zoonotic Spillover and Tropism Shift of a Canine Coronavirus Is Associated with Relaxed Selection and Putative Loss of Function in NTD Subdomain of Spike Protein" Viruses 14, no. 5: 853. https://doi.org/10.3390/v14050853
Citation to related work
MDPI
Has part
Viruses, Vol. 14, No. 5
ADA compliance
For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu