Thumbnail Image
Item

Growth of v-src-transformed cells in serum-free medium through the induction of growth factors

Deangelis, Tiziana
; 
Quong, Andrew
; ; 
Baserga, Renato
Citations
Altmetric:
Genre
Post-print
Date
2012-12-18
Advisor
Committee member
Group
Department
Biology
Subject
Permanent link to this record
http://hdl.handle.net/20.500.12613/7093
Collections
Faculty/Researcher Works
Show all metadata
Files
Thumbnail Image
Morrione-Postprint-2012-12.pdf
Adobe PDF, 677.29 KB
Research Projects
Organizational Units
Journal Issue
DOI
https://doi.org/10.1002/jcp.24303
Abstract
The v-src oncogene is one of only two oncogenes capable of transforming mouse embryo fibroblasts (MEFs) lacking the IGF-IR gene (R-cells). R-/v-src cells grow robustly in the absence of serum, suggesting the hypothesis that they may produce one or more growth factors that would sustain their ability to proliferate in serum-free condition. Using proteomic approaches on serum-free conditioned media derived from v-src-transformed cells, we have identified two growth promoting factors: ostepontin and proliferin. Subsequent experiments have indicated that osteopontin plays a prevalent role in promoting growth of v-src-transformed cells in serum-deprived condition.
Description
Citation
DeAngelis, T., Quong, A., Morrione, A. and Baserga, R. (2013), Growth of v-src-transformed cells in serum-free medium through the induction of growth factors. J. Cell. Physiol., 228: 1482-1486.
Citation to related work
Wiley
This is the peer reviewed version of the following article: DeAngelis, T., Quong, A., Morrione, A. and Baserga, R. (2013), Growth of v-src-transformed cells in serum-free medium through the induction of growth factors. J. Cell. Physiol., 228: 1482-1486.
Has part
Journal of Cellular Physiology, Vol. 228, No. 7
ADA compliance
For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
Embedded videos