Loading...
Technologies Enabling Single-Molecule Super-Resolution Imaging of mRNA
Schnell, Steven J. Yu, Wenlan Saredy, Jason Junod, Samuel Patel, Dhrumil Alkurdi, Abdullah A.
Schnell, Steven J.
Yu, Wenlan
Saredy, Jason
Junod, Samuel
Patel, Dhrumil
Alkurdi, Abdullah A.
Citations
Altmetric:
Genre
Journal article
Date
2022-09-30
Advisor
Committee member
Group
Department
Biology
Permanent link to this record
Collections
Research Projects
Organizational Units
Journal Issue
DOI
https://doi.org/10.3390/cells11193079
Abstract
The transient nature of RNA has rendered it one of the more difficult biological targets for imaging. This difficulty stems both from the physical properties of RNA as well as the temporal constraints associated therewith. These concerns are further complicated by the difficulty in imaging endogenous RNA within a cell that has been transfected with a target sequence. These concerns, combined with traditional concerns associated with super-resolution light microscopy has made the imaging of this critical target difficult. Recent advances have provided researchers the tools to image endogenous RNA in live cells at both the cellular and single-molecule level. Here, we review techniques used for labeling and imaging RNA with special emphases on various labeling methods and a virtual 3D super-resolution imaging technique.
Description
Citation
Tingey M, Schnell SJ, Yu W, Saredy J, Junod S, Patel D, Alkurdi AA, Yang W. Technologies Enabling Single-Molecule Super-Resolution Imaging of mRNA. Cells. 2022; 11(19):3079. https://doi.org/10.3390/cells11193079
Citation to related work
MDPI
Has part
Cells, Vol. 11, No. 19
Special Issue "Technologies and Applications of RNA Imaging"
Special Issue "Technologies and Applications of RNA Imaging"
ADA compliance
For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu