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Analysis Of The Microbiome Associated With Peri-implantitis In Moroccan Patients
Pangam, Tanvi Shyamsundar
Pangam, Tanvi Shyamsundar
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2024-05
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Oral Biology
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http://dx.doi.org/10.34944/dspace/10184
Abstract
Little is known about the microbiome composition associated with peri-implantitis in developing countries. A recent study found a high prevalence of peri-implantitis in a group of Moroccan patients. We hypothesized that a distinct microbiome may be associated with this disease in Moroccan subjects, and the aim of this study was to investigate the composition of the microbiome in peri-implantitis sites and sites without peri-implantitis. The study material consisted of 35 dental patients with dental implants: 22 of these had peri-implantitis, and 13 were without peri-implantitis. Among these subjects, dental plaque samples were collected from 50 peri-implant sites as follows: in the peri-implantitis subjects, 22 samples were from peri-implantitis sites (peri-implantitis patient diseased sites) and 15 samples from sites without peri-implantitis (peri-implantitis patient control sites); and 13 samples from implants from subjects without peri-implantitis (non-peri-implantitis patient control sites). The samples were sequenced for the V1-V3 region of the 16S rRNA gene, and the resultant sequences were classified at the species level using a previously described Blastn-based algorithm. Downstream analysis of the data was performed with Phyloseq, Microbiome, Vegan and MaAsLin packages in R, using a false discovery rate (FDR) cutoff of 0.25. Fifty-six species and 30 genera were identified per sample on average. No significant differences were found between the groups in terms of species richness and alpha diversity. However, beta diversity analysis by PERMANOVA (Adonis) identified a statistically significant difference (FDR=0.024) between the peri-implantitis patient diseased sites and non-peri-implantitis patient control sites. Compared to non-peri-implantitis patient control sites, diseased but not control sites in patients with peri-implantitis showed significantly higher levels of Peptostreptococcus stomatitis and Mogibacterium spp. However, both diseased and control sites in patients with peri-implantitis had higher abundance of Olsenella uli, Atopobium spp. and Actinomyces spp. compared to non-peri-implantitis patient control sites. No differences at FDR ≤ 0.25 were found between diseased and control sites in patients with peri-implantitis, but Porphyromonas endodontalis tended to be elevated in diseased sites while Veillonella parvula tended to increase in control sites. These findings suggest a distinct dysbiotic microbiome is associated with peri-implantitis sites in Moroccan patients.
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