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Streamlined Yeast Colorimetric Reporter Activity Assays Using Scanners and Plate Readers
Serebriiskii, Ilya G. Toby, Garabet G.
Serebriiskii, Ilya G.
Toby, Garabet G.
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Journal article
Date
2000-08
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Cancer and Cellular Biology
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DOI
http://dx.doi.org/10.2144/00292st03
Abstract
Two-hybrid systems have become favored tools for detection and analysis of protein interactions because of their low cost and ease of use compared to biochemical or biophysical interaction technologies. It is possible to augment the utility of two-hybrid systems and derivative systems such as dual-bait two-hybrid systems by adapting strategies that speed the analysis of the relative strength of a series of protein-protein associations. This report describes two simple techniques that employ either a flatbed scanner or a plate reader to quantitate the activity of colorimetric reporters such as LacZ or GusA commonly used in two-hybrid approaches.
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Citation
Serebriiskii IG, Toby GG, Golemis EA. Streamlined Yeast Colorimetric Reporter Activity Assays Using Scanners and Plate Readers. BioTechniques. 2000 Aug;29(2):278-288. doi:10.2144/00292st03
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Future Science Group
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BioTechniques, Vol. 29, Iss. 2
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