Loading...
DIFFERENTIATION OF NEURAL STEM CELL USING SMALL MOLECULES IN 2D AND 3D CULTURE SYSTEM
Shi, Xinglong
Shi, Xinglong
Citations
Altmetric:
Genre
Thesis/Dissertation
Date
2015
Advisor
Committee member
Group
Department
Bioengineering
Permanent link to this record
Collections
Files
Research Projects
Organizational Units
Journal Issue
DOI
http://dx.doi.org/10.34944/dspace/3541
Abstract
The neuronal differentiation of neural stem cells (NSCs) has received much attention due to its potential for the treatment of neurodegenerative diseases (i.e., Parkinson’s and Alzheimer’s diseases). In this regard, discovering compounds that direct differentiation of NSCs is highly required to facilitate therapeutic applications. In this study, we examined various bioactive compounds (SA1, SA2, LiCl, compound B, and DHED) to induce the neuronal differentiation of human neural stem cells (hNSCs). The study was conducted on the cells grown in three dimensional (3D) hydrogel or two dimensional (2D) environment since 3D hydrogel mimics the extracellular matrix and provides physiologically more relevant environment than 2D cell culture system. Three-dimensional (3D) hydrogel systems in this study involve polysaccharides such as alginate and hyaluronic acid. Neuronal differentiation of hNSCs was monitored in genetic level and protein level by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and immunocytochemistry (ICC), respectively. This study will show the effect of bioactive compounds on hNSCs differentiation in 2D and 3D culture systems.
Description
Citation
Citation to related work
Has part
ADA compliance
For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
Embedded videos
License
IN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.