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Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function
Jongwattanapisan, P Terajima, M Miguez, PA Querido, W Nagaoka, H Sumida, N Gurysh, EG Ainslie, KM Pleshko, N Perera, L
Jongwattanapisan, P
Terajima, M
Miguez, PA
Querido, W
Nagaoka, H
Sumida, N
Gurysh, EG
Ainslie, KM
Pleshko, N
Perera, L
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Genre
Journal Article
Date
2018-12-01
Advisor
Committee member
Group
Department
Subject
Animals
Biglycan
Bone Morphogenetic Protein 2
Calcification, Physiologic
Cell Line
Cells, Cultured
Mice
Models, Molecular
Osteogenesis
Peptides
Protein Binding
Protein Conformation
Protein Interaction Domains and Motifs
Rats
Recombinant Fusion Proteins
Signal Transduction
Spectroscopy, Fourier Transform Infrared
Structure-Activity Relationship
Biglycan
Bone Morphogenetic Protein 2
Calcification, Physiologic
Cell Line
Cells, Cultured
Mice
Models, Molecular
Osteogenesis
Peptides
Protein Binding
Protein Conformation
Protein Interaction Domains and Motifs
Rats
Recombinant Fusion Proteins
Signal Transduction
Spectroscopy, Fourier Transform Infrared
Structure-Activity Relationship
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DOI
10.1038/s41598-018-25279-x
Abstract
© 2018 The Author(s). We have reported that recombinant biglycan (BGN) core protein accelerates bone formation in vivo by enhancing bone morphogenetic protein (BMP)-2 function. The purpose of the present study was to identify the specific domain ("effector") within the BGN core protein that facilitates BMP-2 osteogenic function. Thus, we generated various recombinant and synthetic peptides corresponding to several domains of BGN, and tested their effects on BMP-2 functions in vitro. The results demonstrated that the leucine-rich repeats 2-3 domain (LRR2-3) of BGN significantly enhanced the BMP-2 induced Smad1/5/9 phosphorylation, osteogenic gene expression, and alkaline phosphatase activity in myogenic C2C12 cells. Furthermore, addition of LRR2-3 to osteoblastic MC3T3-E1 cells accelerated in vitro mineralization without compromising the quality of the mineral and matrix. These data indicate that LRR2-3 is, at least in part, responsible for BGN's ability to enhance BMP-2 osteogenic function, and it could be useful for bone tissue regeneration.
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Scientific Reports
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