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Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells
Zhang, Yongang Liu, Jinbiao Lin, Yuan Wei, Zhengyu Saribas, Sami Zhu, Yuanjun Li, Fang Wang, Xu
Zhang, Yongang
Liu, Jinbiao
Lin, Yuan
Wei, Zhengyu
Saribas, Sami
Zhu, Yuanjun
Li, Fang
Wang, Xu
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Journal article
Date
2021-07-05
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Pathology and Laboratory Medicine
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https://doi.org/10.3389/fncel.2021.682272
Abstract
Human cerebral organoid (CO) is a three-dimensional (3D) cell culture system that recapitulates the developing human brain. While CO has proved an invaluable tool for studying neurological disorders in a more clinically relevant matter, there have still been several shortcomings including CO variability and reproducibility as well as lack of or underrepresentation of certain cell types typically found in the brain. As the technology to generate COs has continued to improve, more efficient and streamlined protocols have addressed some of these issues. Here we present a novel scalable and simplified system to generate microglia-containing CO (MCO). We characterize the cell types and dynamic development of MCOs and validate that these MCOs harbor microglia, astrocytes, neurons, and neural stem/progenitor cells, maturing in a manner that reflects human brain development. We introduce a novel technique for the generation of embryoid bodies (EBs) directly from induced pluripotent stem cells (iPSCs) that involves simplified steps of transitioning directly from 3D cultures as well as orbital shaking culture in a standard 6-well culture plate. This allows for the generation of MCOs with an easy-to-use system that is affordable and accessible by any general lab.
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Bodnar B, Zhang Y, Liu J, Lin Y, Wang P, Wei Z, Saribas S, Zhu Y, Li F, Wang X, Yang W, Li Q, Ho W-Z and Hu W (2021) Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells. Front. Cell. Neurosci. 15:682272. doi: 10.3389/fncel.2021.682272
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Frontiers Media
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Frontiers in Cellular Neuroscience, Vol. 15
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