This DATSETNAMEreadme.txt file was generated on YYYYMMDD by NAME


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GENERAL INFORMATION
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1. Title of Dataset: Lateralization of Bladder Function in Normal Female Canines 




2. Author Information: Dania Giaddui, Danielle S. Porreca, Ekta Tiwari, Nagat A. Frara, Lucas J. Hobson, Mary F. Barbe, Alan S. Braverman, Justin M. Brown, Michel A. Pontari, Michael R. Ruggieri, Sr.






  Principal Investigator Contact Information
        Name: Michael R. Ruggieri, Sr, PhD
           Institution: Lewis Katz School of Medicine, Temple University
           Address: 3500 North Broad Street, Philadelphia, PA 19140


           Email: Michael.Ruggieri@temple.edu
           ORCID:


  Associate or Co-investigator Contact Information
        Name: Mary F Barbe, PhD
           Institution: Lewis Katz School of Medicine, Temple University
           Address: 3500 North Broad Street, Philadelphia, PA 19140
           Email: mary.barbe@temple.edu
           ORCID: 0000-0002-5235-9803


  Associate or Co-investigator Contact Information
           Name:
           Institution:
           Address:
           Email:
           ORCID:




3. Date of data collection (single date, range, approximate date): 2014-2021


4. Geographic location of data collection: Philadelphia, PA, United States 


5. Information about funding sources or sponsorship that supported the collection of the data:
Research reported in this publication was supported by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health under Award Number R01NS070267 to Dr. Michael R. Ruggieri and Dr. Mary F. Barbe.


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SHARING/ACCESS INFORMATION
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1. Licenses/restrictions placed on the data, or limitations of reuse: Unrestricted


2. Recommended citation for the data: Not yet accepted: Lateralization of Bladder Function in Normal Female Canines, Giaddui et al, 2021, PlosOne


3. Citation for and links to publications that cite or use the data:


4. Links to other publicly accessible locations of the data:


5. Links/relationships to ancillary or related data sets: 


6. Was data derived from another source? No
           If yes, list source(s):




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DATA & FILE OVERVIEW
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1. File list (filenames, directory structure (for zipped files) and brief description of all data files, add additional entries as necessary):


   A. Filename:     Data-for-Lateralization-of-Bladder-Function-in-Normal-Female-Canines_Sept 1_2021.xlsx   
      Short description:        


An excel file is provided that includes the raw data for analyses in a paper entitled: Lateralization of Bladder Function in Normal Female Canines, by Giaddui et al. 

B.   Filename:  Data-for-Lateralization-of-Bladder-Function-in-Normal-Female-Canines_Sept 1_2021.csv
Short description: Csv copy of File A




2. Relationship between files, if important for context:        
Files B is a csv copy of file A, an Excel workbook.




3. Additional related data collected that was not included in the current data package:


N/A




4. Are there multiple versions of the dataset? No








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METHODOLOGICAL INFORMATION
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1. Description of methods used for collection/generation of data: <Include links or references to publications or other documentation containing experimental design or protocols used in data collection>


Spinal root & pelvic nerve’s anterior vesical branch functional electrical stimulation


Prior to surgery, animals received 6 mg/kg IV of propofol to allow for the insertion of the endotracheal tube. Anesthesia was then maintained using isoflurane at 2-3% maximum alveolar concentration with oxygen. All animals underwent lumbosacral laminectomy of L6-S3 vertebrae to expose the lower spinal cord and spinal roots, and to identify nerves via electrical stimulation, as previously described (Gomez-Amaya et al., 2015; Ruggieri et al., 2006). Bladder pressure monitoring was obtained by catheterizing the urethra with a double balloon catheter connected to external pressure transducers for the continuous recording of bladder and urethral pressures and for bladder filling with a syringe pump.  Balloon catheters were also inserted into the rectum at the level of the bladder dome interfaced with pressure transducers for the continuous recording of rectal pressures. The pressure transducers used were Becton Dickinson Dtxplus Model DT-XX with a 50 Hz low pass filter and a sampling rate of 4/second which were calibrated immediately prior to each procedure. The bladder capacity (defined as the volume inducing a arked increase in the slope of the volume-pressure curve) was determined with 3 successive filling cystometrograms using normal saline (30 mL/min) and the bladder was then emptied and filled to half of its capacity.  Because of the urethral catheter, the bladder was not able to empty during the stimulations and thus isometric bladder contractions were recorded.  The time between setting the bladder volume at half of its capacity and the stimulations were consistent between different animals such that the bladder volume was consistent between different animals.  
Intraoperative electrical stimulation of left and right L6 through S3 spinal roots and nerves within the pelvic plexus of the bladder, specifically the anterior vesical branch of the pelvic nerve emerging from the pelvic plexus towards the bladder body was performed.  Handheld monopolar or bipolar electrodes (tip diameter  0.5mm, Medtronic Xomed Inc.) with currents of 0.5 – 10 mA, a frequency of 20-Hz, and a pulse duration of 0.2 msec were used.  These electrical stimulations were generated using a Grass Astro-Med model S88X stimulator interfaced stimulus isolation unit (SIU-C)  for constant current square pulse trains of 2-5 second durations.   If a monopolar electrode was used, the ground was connected over the chest skin. Changes in detrusor pressure (defined as rectal pressure subtracted from intravesical pressure) were continuously recorded with  a PowerLab multichannel data acquisition system and LabChart software (ADInstruments, Colorado Springs, CO). Strength of nerve-evoked bladder contractions resulting from electrical stimulations were derived from differences between the resting baseline pressure and the peak pressure obtained during continuous stimulation. Intravesical, rectal and detrusor pressure recording for both the left and the right side of S2 spinal root stimulation are shown in Figure 1.  To assure that fatigue of the detrusor muscle was not induced, multiple stimulations were separated by an average rest period of 1-2 minutes between stimulations.


Gomez-Amaya, S. M., Barbe, M. F., Brown, J. M., Lamarre, N. S., Braverman, A. S., Massicotte, V. S., & Ruggieri, M. R., Sr. (2015). Bladder reinnervation using a primarily motor donor nerve (femoral nerve branches) is functionally superior to using a primarily sensory donor nerve (genitofemoral nerve). J Urol, 193(3), 1042-1051. doi:10.1016/j.juro.2014.07.095


Ruggieri, M. R., Braverman, A. S., D'Andrea, L., Simpkiss, B., Kozin, S. H., Pontari, M. A., . . . Barbe, M. F. (2006). Functional reinnervation of the canine bladder after spinal root transection and immediate end-on-end repair. J Neurotrauma, 23(7), 1125-1136. doi:10.1089/neu.2006.23.1125


2. Methods for processing the data: <describe how the submitted data were generated from the raw or collected data>
Statistical analysis 
The dominant side for functional innervation of the bladder in each animal was determined by calculating the percent difference between the left and right spinal root stimulation at spinal levels known to contribute to bladder function, as well as that of left and right anterior vesical branch of the pelvic nerve. We evaluated for left- or right-sided functional lateralization using two different parameters: either 25% or 10% difference when comparing sides. If differences were within the defined range, bladders were considered as bilaterally innervated functionally. The percent difference was calculated by taking the difference between left and right stimulations for each spinal root or pelvic nerve and dividing it by the average of both stimulations. For Figure 2A-F and Figure 3, the maximum detrusor pressure of the right side was plotted on the x-axis. Based on the percent differences, the maximum detrusor pressure of the left side was allocated to either left, right or bilateral on the y-axis with different symbols as shown on the Figures. All data were plotted along with a line of equivalence (y=x). Spearman and Pearson correlation tests (one tailed and two tailed) were performed using GraphPad Prism 8.4 (La Jolla, CA) for each spinal root and for pelvic nerve stimulation. A p-value of < 0.05 was considered to be statistically significant.


3. Software- or Instrument-specific information needed to interpret the data, including software and hardware version numbers:


Indicated above, in points 1 and 2, 


4. Standards and calibration information, if appropriate:


Indicated above, in points 1 and 2, 


5. Environmental/experimental conditions:


Indicated above, in points 1 and 2, 


6. Describe any quality-assurance procedures performed on the data:


Indicated above, in points 1 and 2, 


7. People involved with sample collection, processing, analysis and/or submission:


Dania Giaddui, Danielle S. Porreca, Ekta Tiwari, Nagat A. Frara, Lucas J. Hobson, Mary F. Barbe, Alan S. Braverman, Justin M. Brown, Michel A. Pontari, Michael R. Ruggieri, Sr.


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DATA-SPECIFIC INFORMATION FOR:  Data-for-Lateralization-of-Bladder-Function-in-Normal-Female-Canines_Sept 1_2021.xlsx
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Data for “ Data for Lateralization of Bladder Function in Normal Female Canines_Sept 1_2021.xlsx” is provided as an excel file.


The remaining information is explained in the Methodological Information Section


1. Number of variables:


2. Number of cases/rows: 


   
3. Variable List, defining any abbreviations, units of measure, codes or symbols used:


    Example. Name: Gender 
             Description: Gender of respondent
                    1 = Male
                    2 = Female
4. Missing data codes:
        Code/symbol        Definition
        Code/symbol        Definition




5. Specialized formats or other abbreviations used:


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DATA-SPECIFIC INFORMATION FOR CODE: [FILENAME]  (Repeated as necessary)
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1. Language used:


2. Software necessary for editing and running:


3. Purpose of code:


4. Overview of operation of code (inputs, outputs, etc.):








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DATA-SPECIFIC INFORMATION FOR VISUALIZATION: [FILENAME]  (Repeated as necessary)
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1. Kinds of visualization: 


2. Program used to generate visualization:


3. Data file(s) used to generate visualization:


4. Describe the process used to generate the visualization: